Novel Putative Nicotinic Acetylcholine Receptor Subunit Genes, D5, D6 and D7, in Drosophila melanogaster Identify a New and Highly Conserved Target of Adenosine Deaminase Acting on RNA-Mediated A-to-I Pre-mRNA Editing

Corresponding author: D. B. Sattelle, Department of Human Anatomy and Genetics, University of Oxford, S. Parks Rd., Oxford OX1 3QX, United Kingdom., david.sattelle{at}anat.ox.ac.uk (E-mail)

Communicating editor: A. J. LOPEZ

Genome analysis of the fruit fly Drosophila melanogaster reveals three new ligand-gated ion channel subunits with the characteristic YXCC motif found only in -type nicotinic acetylcholine receptor subunits. The subunits are designated D5, D6, and D7. Cloning of the D5 embryonic cDNAs reveals an atypically large N terminus, part of which is without identifiable sequence motifs and is specified by two polymorphic alleles. Embryonic clones from D6 contain multiple variant transcripts arising from alternative splicing as well as A-to-I pre-mRNA editing. Alternative splicing in D6 involves exons encoding nAChR functional domains. The D6 transcript is a target of the Drosophila adenosine deaminase acting on RNA (dADAR). This is the first case for any organism where a nAChR gene is the target of mRNA editing. Seven adenosines could be modified in the extracellular ligand-binding region of D6, four of which are also edited in the D6 ortholog in the tobacco budworm Heliothis virescens. The conservation of an editing site between the insect orders Diptera and Lepidoptera makes nAChR editing the most evolutionarily conserved invertebrate RNA editing site so far described. These findings add to our understanding of nAChR subunit diversity, which is increased and regulated by mechanisms acting at the genomic and mRNA levels.