help button home button Genetics AJP: Renal Phys
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mongelard, F.
Right arrow Articles by Corces, V. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mongelard, F.
Right arrow Articles by Corces, V. G.
Genetics, Vol. 160, 1481-1487, April 2002, Copyright © 2002

Trans-splicing as a Novel Mechanism to Explain Interallelic Complementation in Drosophila

Fabien Mongelarda, Mariano Labradora, Ellen M. Baxtera, Tatiana I. Gerasimovaa, and Victor G. Corcesa
a Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218

Corresponding author: Victor G. Corces, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218., corces{at}jhu.edu (E-mail)

Communicating editor: S. HENIKOFF

Two mutant alleles of the same gene, each located in one of the two homologous chromosomes, may in some instances restore the wild-type function of the gene. This is the case with certain combinations of mutant alleles in the mod(mdg4) gene. This gene encodes several different proteins, including Mod(mdg4)2.2, a component of the gypsy insulator. This protein is encoded by two separate transcription units that can be combined in a trans-splicing reaction to form the mature Mod(mdg4)2.2-encoding RNA. Molecular characterization of complementing alleles shows that they affect the two different transcription units. Flies homozygous for each allele are missing the Mod(mdg4)2.2 protein, whereas wild-type trans-heterozygotes are able to synthesize almost normal levels of the Mod(mdg4)2.2 product. This protein is functional as judged by its ability to form a functional insulator complex. The results suggest that the interallelic complementation in the mod(mdg4) gene is a consequence of trans-splicing between two different mutant transcripts. A conclusion from this observation is that the trans-splicing reaction that takes place between transcripts produced on two different mutant chromosomes ensures wild-type levels of functional protein.




This article has been cited by other articles:


Home page
 GeneticsHome page
M. Soltani-Bejnood, S. E. Thomas, L. Villeneuve, K. Schwartz, C.-s. Hong, and B. D. McKee
Role of the mod(mdg4) Common Region in Homolog Segregation in Drosophila Male Meiosis
Genetics, May 1, 2007; 176(1): 161 - 180.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Gabler, M. Volkmar, S. Weinlich, A. Herbst, P. Dobberthien, S. Sklarss, L. Fanti, S. Pimpinelli, H. Kress, G. Reuter, et al.
Trans-splicing of the mod(mdg4) Complex Locus Is Conserved Between the Distantly Related Species Drosophila melanogaster and D. virilis
Genetics, February 1, 2005; 169(2): 723 - 736.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Melnikova, F. Juge, N. Gruzdeva, A. Mazur, G. Cavalli, and P. Georgiev
Interaction between the GAGA factor and Mod(mdg4) proteins promotes insulator bypass in Drosophila
PNAS, October 12, 2004; 101(41): 14806 - 14811.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Karakozova, E. Savitskaya, L. Melnikova, A. Parshikov, and P. Georgiev
The Mod(mdg4) Component of the Su(Hw) Insulator Inserted in the P Transposon Can Repress Its Mobility in Drosophila melanogaster
Genetics, July 1, 2004; 167(3): 1275 - 1280.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Horiuchi, E. Giniger, and T. Aigaki
Alternative trans-splicing of constant and variable exons of a Drosophila axon guidance gene, lola
Genes & Dev., October 15, 2003; 17(20): 2496 - 2501.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
M. Labrador and V. G. Corces
Extensive Exon Reshuffling Over Evolutionary Time Coupled to Trans-Splicing in Drosophila
Genome Res., October 1, 2003; 13(10): 2220 - 2228.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
K. Byrd and V. G. Corces
Visualization of chromatin domains created by the gypsy insulator of Drosophila
J. Cell Biol., August 18, 2003; 162(4): 565 - 574.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Genetics Society of America.