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The Mitochondrial Nucleoid Protein, Mgm101p, of Saccharomyces cerevisiae Is Involved in the Maintenance of 
+ and ori/rep-Devoid Petite Genomes but Is Not Required for Hypersuppressive - mtDNA
Xiao Ming Zuoa,
G. Desmond Clark-Walkera, and
Xin Jie Chena
a Molecular Genetics and Evolution Group, Research School of Biological Sciences, The Australian National University, Canberra, ACT 2601, Australia
Corresponding author: Xin Jie Chen, Research School of Biological Sciences, The Australian National University, GPO Box 475, Canberra City, ACT 2601, Australia., chen{at}rsbs.anu.edu.au (E-mail)
Communicating editor: L. PILLUS
+ genome because meiotic segregants, with a disrupted mgm101 allele, cannot undergo more than 10 divisions on glycerol medium. Quantitative analysis of mtDNA copy number in a + strain carrying a temperature-sensitive allele, mgm101-1, revealed that the amount of mtDNA is halved each cell division upon a shift to the restrictive temperature. These data suggest that mtDNA replication is rapidly blocked in cells lacking MGM101. However, a small proportion of meiotic segregants, disrupted in MGM101, have - genomes that are stably maintained. Interestingly, all surviving - mtDNAs contain an ori/rep sequence. Disruption of MGM101 in hypersuppressive (HS) strains does not have a significant effect on the propagation of HS - mtDNA. However, in petites lacking an ori/rep, disruption of MGM101 leads to either a complete loss or a dramatically decreased stability of mtDNA. This discriminatory effect of MGM101 suggests that replication of + and ori/rep-devoid - mtDNAs is carried out by the same process. By contrast, the persistence of ori/rep-containing mtDNA in HS petites lacking MGM101 identifies a distinct replication pathway. The alternative mtDNA replication mechanism provided by ori/rep is independent of mitochondrial RNA polymerase encoded by RPO41 as a HS - genome is stably maintained in a mgm101, rpo41 double mutant.
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