Genetics, Vol. 160, 1035-1050, March 2002, Copyright © 2002

A Misexpression Study Examining Dorsal Thorax Formation in Drosophila melanogaster

María Teresa Peña-Rangela,b, Isabel Rodriguezc, and Juan Rafael Riesgo-Escovara
a Department of Developmental Neurobiology and Neurophysiology, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro, 76230, México,
b Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Querétaro, 76010, México
c Centro de Biologia Molecular "Severo Ochoa, " Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Cientificas, 28049 Madrid, España

Corresponding author: Juan Rafael Riesgo-Escovar, Instituto de Neurobiología, UNAM, Futbol #149, Col. Country Club Churubusco, México, D. F., 04220, México., riesgo{at}mail.cnb.unam.mx (E-mail)

Communicating editor: T. SCHÜPBACH

We studied thorax formation in Drosophila melanogaster using a misexpression screen with EP lines and thoracic Gal4 drivers that provide a genetically sensitized background. We identified 191 interacting lines showing alterations of thoracic bristles (number and/or location), thorax and scutellum malformations, lethality, or suppression of the thoracic phenotype used in the screen. We analyzed these lines and showed that known genes with different functional roles (selector, prepattern, proneural, cell cycle regulation, lineage restriction, signaling pathways, transcriptional control, and chromatin organization) are among the modifier lines. A few lines have previously been identified in thorax formation, but others, such as chromatin-remodeling complex genes, are novel. However, most of the interacting loci are uncharacterized, providing a wealth of new genetic data. We also describe one such novel line, poco pelo (ppo), where both misexpression and loss-of-function phenotypes are similar: loss of bristles and scutellum malformation.




This article has been cited by other articles:


Home page
 GeneticsHome page
M. Stofanko, S. Y. Kwon, and P. Badenhorst
A Misexpression Screen to Identify Regulators of Drosophila Larval Hemocyte Development
Genetics, September 1, 2008; 180(1): 253 - 267.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Khokhar, N. Chen, J.-P. Yuan, Y. Li, G. N. Landis, G. Beaulieu, H. Kaur, and J. Tower
Conditional Switches for Extracellular Matrix Patterning in Drosophila melanogaster
Genetics, March 1, 2008; 178(3): 1283 - 1293.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
T. Zhao, T. Gu, H. C. Rice, K. L. McAdams, K. M. Roark, K. Lawson, S. A. Gauthier, K. L. Reagan, and R. S. Hewes
A Drosophila Gain-of-Function Screen for Candidate Genes Involved in Steroid-Dependent Neuroendocrine Cell Remodeling
Genetics, February 1, 2008; 178(2): 883 - 901.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. Molnar, A. Lopez-Varea, R. Hernandez, and J. F. de Celis
A Gain-of-Function Screen Identifying Genes Required for Vein Formation in the Drosophila melanogaster Wing
Genetics, November 1, 2006; 174(3): 1635 - 1659.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
X. Sun, T. Morozova, and M. Sonnenfeld
Glial and Neuronal Functions of the Drosophila Homolog of the Human SWI/SNF Gene ATR-X (DATR-X) and the jing Zinc-Finger Gene Specify the Lateral Positioning of Longitudinal Glia and Axons
Genetics, July 1, 2006; 173(3): 1397 - 1415.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. S. Hammonds and J. W. Fristrom
Mutational Analysis of Stubble-stubbloid Gene Structure and Function in Drosophila Leg and Bristle Morphogenesis
Genetics, March 1, 2006; 172(3): 1577 - 1593.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Muller, S. J. Kugler, A. Preiss, D. Maier, and A. C. Nagel
Genetic Modifier Screens on Hairless Gain-of-Function Phenotypes Reveal Genes Involved in Cell Differentiation, Cell Growth and Apoptosis in Drosophila melanogaster
Genetics, November 1, 2005; 171(3): 1137 - 1152.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Singh, J. Chan, J. J. Chern, and K.-W. Choi
Genetic Interaction of Lobe With Its Modifiers in Dorsoventral Patterning and Growth of the Drosophila Eye
Genetics, September 1, 2005; 171(1): 169 - 183.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
M. J. Williams, I. Ando, and D. Hultmark
Drosophila melanogaster Rac2 is necessary for a proper cellular immune response
Genes Cells, August 1, 2005; 10(8): 813 - 823.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. T. Collins and S. M. Cohen
A Genetic Screen in Drosophila for Identifying Novel Components of the Hedgehog Signaling Pathway
Genetics, May 1, 2005; 170(1): 173 - 184.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. Weber, N. Johnson, D. Champlin, and A. Patty
Many P-Element Insertions Affect Wing Shape in Drosophila melanogaster
Genetics, March 1, 2005; 169(3): 1461 - 1475.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. I. Ivanov, A. C. Rovescalli, P. Pozzi, S. Yoo, B. Mozer, H.-P. Li, S.-H. Yu, H. Higashida, V. Guo, M. Spencer, et al.
Genes required for Drosophila nervous system development identified by RNA interference
PNAS, November 16, 2004; 101(46): 16216 - 16221.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. Raymond, E. Bergeret, A. Avet-Rochex, R. Griffin-Shea, and M.-O. Fauvarque
A screen for modifiers of RacGAP(84C) gain-of-function in the Drosophila eye revealed the LIM kinase Cdi/TESK1 as a downstream effector of Rac1 during spermatogenesis
J. Cell Sci., June 1, 2004; 117(13): 2777 - 2789.
[Abstract] [Full Text] [PDF]