Genetics, Vol. 160, 393-405, February 2002, Copyright © 2002

Genetic Interactions of Yeast Eukaryotic Translation Initiation Factor 5A (eIF5A) Reveal Connections to Poly(A)-Binding Protein and Protein Kinase C Signaling

Sandro R. Valentinia,b, Jason M. Casolaria, Carla C. Oliveirac, Pamela A. Silvera, and Anne E. McBridea
a Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, and Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115,
b Department of Biological Sciences, School of Pharmacy, São Paulo State University, Araraquara, SP, 14801-902, Brazil
c Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508-900, Brazil

Corresponding author: Pamela A. Silver, 44 Binney St., SM922, Boston, MA 02115., pamela_silver{at}dfci.harvard.edu (E-mail)

Communicating editor: L. PILLUS

The highly conserved eukaryotic translation initiation factor eIF5A has been proposed to have various roles in the cell, from translation to mRNA decay to nuclear protein export. To further our understanding of this essential protein, three temperature-sensitive alleles of the yeast TIF51A gene have been characterized. Two mutant eIF5A proteins contain mutations in a proline residue at the junction between the two eIF5A domains and the third, strongest allele encodes a protein with a single mutation in each domain, both of which are required for the growth defect. The stronger tif51A alleles cause defects in degradation of short-lived mRNAs, supporting a role for this protein in mRNA decay. A multicopy suppressor screen revealed six genes, the overexpression of which allows growth of a tif51A-1 strain at high temperature; these genes include PAB1, PKC1, and PKC1 regulators WSC1, WSC2, and WSC3. Further results suggest that eIF5A may also be involved in ribosomal synthesis and the WSC/PKC1 signaling pathway for cell wall integrity or related processes.




This article has been cited by other articles:


Home page
 J Exp BotHome page
J. Song and S. Tachibana
Loss of viability of tomato pollen during long-term dry storage is associated with reduced capacity for translating polyamine biosynthetic enzyme genes after rehydration
J. Exp. Bot., December 7, 2007; (2007) erm280v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Feng, Q. Chen, J. Feng, J. Zhang, X. Yang, and J. Zuo
Functional Characterization of the Arabidopsis Eukaryotic Translation Initiation Factor 5A-2 That Plays a Crucial Role in Plant Growth and Development by Regulating Cell Division, Cell Growth, and Cell Death
Plant Physiology, July 1, 2007; 144(3): 1531 - 1545.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Wagner and G. Klug
An Archaeal Protein with Homology to the Eukaryotic Translation Initiation Factor 5A Shows Ribonucleolytic Activity
J. Biol. Chem., May 11, 2007; 282(19): 13966 - 13976.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. R. Kang, Y. S. Kim, E. C. Wolff, and M. H. Park
Specificity of the Deoxyhypusine Hydroxylase-Eukaryotic Translation Initiation Factor (eIF5A) Interaction: IDENTIFICATION OF AMINO ACID RESIDUES OF THE ENZYME REQUIRED FOR BINDING OF ITS SUBSTRATE, DEOXYHYPUSINE-CONTAINING eIF5A
J. Biol. Chem., March 16, 2007; 282(11): 8300 - 8308.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Huang, D. S. Higginson, L. Hester, M. H. Park, and S. H. Snyder
Neuronal growth and survival mediated by eIF5A, a polyamine-modified translation initiation factor
PNAS, March 6, 2007; 104(10): 4194 - 4199.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Schrader, C. Young, D. Kozian, R. Hoffmann, and F. Lottspeich
Temperature-sensitive eIF5A Mutant Accumulates Transcripts Targeted to the Nonsense-mediated Decay Pathway
J. Biol. Chem., November 17, 2006; 281(46): 35336 - 35346.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
M. H. Park
The Post-Translational Synthesis of a Polyamine-Derived Amino Acid, Hypusine, in the Eukaryotic Translation Initiation Factor 5A (eIF5A)
J. Biochem., February 1, 2006; 139(2): 161 - 169.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. F. Zanelli and S. R. Valentini
Pkc1 Acts Through Zds1 and Gic1 to Suppress Growth and Cell Polarity Defects of a Yeast eIF5A Mutant
Genetics, December 1, 2005; 171(4): 1571 - 1581.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Windgassen, D. Sturm, I. J. Cajigas, C. I. Gonzalez, M. Seedorf, H. Bastians, and H. Krebber
Yeast Shuttling SR Proteins Npl3p, Gbp2p, and Hrb1p Are Part of the Translating mRNPs, and Npl3p Can Function as a Translational Repressor
Mol. Cell. Biol., December 1, 2004; 24(23): 10479 - 10491.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. K. Chattopadhyay, C. W. Tabor, and H. Tabor
Spermidine but not spermine is essential for hypusine biosynthesis and growth in Saccharomyces cerevisiae: Spermine is converted to spermidine in vivo by the FMS1-amine oxidase
PNAS, November 25, 2003; 100(24): 13869 - 13874.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Ober, R. Harms, L. Witte, and T. Hartmann
Molecular Evolution by Change of Function. ALKALOID-SPECIFIC HOMOSPERMIDINE SYNTHASE RETAINED ALL PROPERTIES OF DEOXYHYPUSINE SYNTHASE EXCEPT BINDING THE eIF5A PRECURSOR PROTEIN
J. Biol. Chem., April 4, 2003; 278(15): 12805 - 12812.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. C.M. Kwok and J. T.Y. Wong
Cellulose Synthesis Is Coupled to Cell Cycle Progression at G1 in the Dinoflagellate Crypthecodinium cohnii
Plant Physiology, April 1, 2003; 131(4): 1681 - 1691.
[Abstract] [Full Text] [PDF]