Genetics, Vol. 159, 981-985, November 2001, Copyright © 2001

Isolation of X and Y Chromosome-Specific DNA Markers From a Liverwort, Marchantia polymorpha, by Representational Difference Analysis

Masaki Fujisawaa, Kiwako Hayashia, Tomohisa Nishioa, Tomoyuki Bandoa, Sachiko Okadaa, Katsuyuki T. Yamatoa, Hideya Fukuzawaa, and Kanji Ohyamaa
a Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan

Corresponding author: Kanji Ohyama, Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan., kohyama{at}lif.kyoto-u.ac.jp (E-mail)

Communicating editor: D. CHARLESWORTH

The liverwort Marchantia polymorpha has X and Y chromosomes in the respective female and male haploids. Here we report the successful exploitation of representational difference analyses to isolate DNA markers for the sex chromosomes. Two female-specific and six male-specific DNA fragments were genetically confirmed to originate from the X and Y chromosomes, respectively.




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