Genetics, Vol. 158, 1825-1827, August 2001, Copyright © 2001

Identification of a Mutant Locus by Noncomplementation of a Transposon Insertion Library in Saccharomyces cerevisiae

Heather A. Wiatrowskia and Marian Carlsona
a Departments of Genetics and Development and Microbiology, Columbia University, New York, New York 10032

Corresponding author: Marian Carlson, Columbia University, 701 W. 168th St., HSC922, New York, NY 10032., mbc1{at}columbia.edu (E-mail)

Communicating editor: F. WINSTON

We describe a new approach for identifying the gene corresponding to a mutation in Saccharomyces cerevisiae. A library of mTn-lacZ/LEU2 insertions is tested for failure to complement the mutation, and the noncomplementing insertion is used to obtain sequence. This approach offers an alternative to cloning by complementation with a plasmid library.




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