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Genetic Analysis of the Drosophila Gs
Gene
William J. Wolfganga,
Ashwini Hoskotea,
Ian J. H. Robertsa,
Shannon Jacksona, and
Michael Fortea
a Vollum Institute, L474 Oregon Health Sciences University, Portland, Oregon 97201
Corresponding author: Michael Forte, Vollum Institute, L474, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Rd., Portland, OR 97201., forte{at}ohsu.edu (E-mail)
Communicating editor: T. C. KAUFMAN
, the subsequent stimulation of adenylyl cyclase, production of cAMP, activation of protein kinase A (PKA), and the phosphorylation of substrates that control a wide variety of cellular responses. Here, we report the identification of "loss-of-function" mutations in the Drosophila Gs gene (dgs). Seven mutants have been identified that are either complemented by transgenes representing the wild-type dgs gene or contain nucleotide sequence changes resulting in the production of altered Gs protein. Examination of mutant alleles representing loss-of-Gs function indicates that the phenotypes generated do not mimic those created by mutational elimination of PKA. These results are consistent with the conclusion reached in previous studies that activation of PKA, at least in these developmental contexts, does not depend on receptor-mediated increases in intracellular cAMP, in contrast to the predictions of models developed primarily on the basis of studies in cultured cells.
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