Genetics, Vol. 158, 715-726, June 2001, Copyright © 2001

SSCP Analysis of cDNA Markers Provides a Dense Linkage Map of the Aedes aegypti Genome

Ruth E. Fultona, Michael L. Salaseka, Nancy M. DuTeaua, and William C. Black, IVa
a Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523

Corresponding author: William C. Black, IV, Department of Microbiology, Colorado State University, Fort Collins, CO 80523., wcb4{at}lamar.colostate.edu (E-mail)

Communicating editor: G. A. CHURCHILL

An intensive linkage map of the yellow fever mosquito, Aedes aegypti, was constructed using single-strand conformation polymorphism (SSCP) analysis of cDNA markers to identify single nucleotide polymorphisms (SNPs). A total of 94 A. aegypti cDNAs were downloaded from GenBank and primers were designed to amplify fragments <500 bp in size. These primer pairs amplified 94 loci, 57 (61%) of which segregated in a single F1 intercross family among 83 F2 progeny. This allowed us to produce a dense linkage map of one marker every 2 cM distributed over a total length of 134 cM. Many A. aegypti cDNAs were highly similar to genes in the Drosophila melanogaster genome project. Comparative linkage analysis revealed areas of synteny between the two species. SNP polymorphisms are abundant in A. aegypti genes and should prove useful in both population genetics and mapping studies.




This article has been cited by other articles:


Home page
 Am J Trop Med HygHome page
J. Wong, F. Tripet, J. L. Rasgon, G. C. Lanzaro, and T. W. Scott
SSCP Analysis of scnDNA for Genetic Profiling of Aedes aegypti
Am J Trop Med Hyg, October 1, 2008; 79(4): 511 - 517.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. Saavedra-Rodriguez, C. Strode, A. Flores Suarez, I. Fernandez Salas, H. Ranson, J. Hemingway, and W. C. Black IV
Quantitative Trait Loci Mapping of Genome Regions Controlling Permethrin Resistance in the Mosquito Aedes aegypti
Genetics, October 1, 2008; 180(2): 1137 - 1152.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Zhong, D. M. Menge, E. A. Temu, H. Chen, and G. Yan
Amplified Fragment Length Polymorphism Mapping of Quantitative Trait Loci for Malaria Parasite Susceptibility in the Yellow Fever Mosquito Aedes aegypti
Genetics, July 1, 2006; 173(3): 1337 - 1345.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. E. Bennett, D. Flick, K. H. Fleming, R. Jochim, B. J. Beaty, and W. C. Black IV
Quantitative Trait Loci That Control Dengue-2 Virus Dissemination in the Mosquito Aedes aegypti
Genetics, May 1, 2005; 170(1): 185 - 194.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. R. Anderson, J. R. Schneider, P. R. Grimstad, and D. W. Severson
Quantitative Genetics of Vector Competence for La Crosse Virus and Body Size in Ochlerotatus hendersoni and Ochlerotatus triseriatus Interspecific Hybrids
Genetics, March 1, 2005; 169(3): 1529 - 1539.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Solignac, D. Vautrin, E. Baudry, F. Mougel, A. Loiseau, and J.-M. Cornuet
A Microsatellite-Based Linkage Map of the Honeybee, Apis mellifera L.
Genetics, May 1, 2004; 167(1): 253 - 262.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
D. W. Severson, B. deBruyn, D. D. Lovin, S. E. Brown, D. L. Knudson, and I. Morlais
Comparative Genome Analysis of the Yellow Fever Mosquito Aedes aegypti with Drosophila melanogaster and the Malaria Vector Mosquito Anopheles gambiae
J. Hered., March 1, 2004; 95(2): 103 - 113.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
E. W. CHAMBERS, D. D. LOVIN, and D. W. SEVERSON
UTILITY OF COMPARATIVE ANCHOR-TAGGED SEQUENCES AS PHYSICAL ANCHORS FOR COMPARATIVE GENOME ANALYSIS AMONG THE CULICIDAE
Am J Trop Med Hyg, July 1, 2003; 69(1): 98 - 104.
[Abstract] [Full Text] [PDF]