Genetics, Vol. 158, 613-625, June 2001, Copyright © 2001

The Saccharomyces cerevisiae Small GTPase, Gsp1p/Ran, Is Involved in 3' Processing of 7S-to-5.8S rRNA and in Degradation of the Excised 5'-A0 Fragment of 35S Pre-rRNA, Both of Which Are Carried Out by the Exosome

Nobuhiro Suzukia,b, Eishi Noguchia, Nobutaka Nakashimaa, Masaya Okia, Tomoyuki Ohbaa, Alan Tartakoffc, Masamichi Ohishib, and Takeharu Nishimotoa
a Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812-8582, Japan,
b Oral and Maxillofacial Surgery Advanced Course, Division of Dental Science, Graduate School, Kyushu University, Fukuoka 812-8582, Japan
c Department of Pathology and Cell Biology Program, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-2622

Corresponding author: Takeharu Nishimoto, Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, 3-1-1 Maedashi, Higashiku, Fukuoka 812-8582, Japan., tnishi{at}molbiol.med.kyushu-u.ac.jp (E-mail)

Communicating editor: A. G. HINNEBUSCH

Dis3p, a subunit of the exosome, interacts directly with Ran. To clarify the relationship between the exosome and the RanGTPase cycle, a series of temperature-sensitive Saccharomyces cerevisiae dis3 mutants were isolated and their 5.8S rRNA processing was compared with processing in strains with mutations in a S. cerevisiae Ran homologue, Gsp1p. In both dis3 and gsp1 mutants, 3' processing of 7S-to-5.8S rRNA was blocked at three identical sites in an allele-specific manner. In contrast, the 5' end of 5.8S rRNA was terminated normally in gsp1 and in dis3. Inhibition of 5.8S rRNA maturation in gsp1 was rescued by overexpression of nuclear exosome components Dis3p, Rrp4p, and Mtr4p, but not by a cytoplasmic exosome component, Ski2p. Furthermore, gsp1 and dis3 accumulated the 5'-A0 fragment of 35S pre-rRNA, which is also degraded by the exosome, and the level of 27S rRNA was reduced. Neither 5.8S rRNA intermediates nor 5'-A0 fragments were observed in mutants defective in the nucleocytoplasmic transport, indicating that Gsp1p regulates rRNA processing through Dis3p, independent of nucleocytoplasmic transport.




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