Genetics, Vol. 158, 95-107, May 2001, Copyright © 2001

Saccharomyces cerevisiae SMT4 Encodes an Evolutionarily Conserved Protease With a Role in Chromosome Condensation Regulation

Alexander V. Strunnikova, L. Aravindb, and Eugene V. Kooninb
a National Institute of Child Health and Human Development, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20892
b National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20892

Corresponding author: Alexander V. Strunnikov, NIH, NICHD, LGRD, 18T Library Dr., Rm. 106, Bethesda, MD 20892., strunnik{at}box-s.nih.gov (E-mail)

Communicating editor: S. HENIKOFF

In a search for regulatory genes affecting the targeting of the condensin complex to chromatin in Saccharomyces cerevisiae, we identified a member of the adenovirus protease family, SMT4. SMT4 overexpression suppresses the temperature-sensitive conditional lethal phenotype of smc2-6, but not smc2-8 or smc4-1. A disruption allele of SMT4 has a prominent chromosome phenotype: impaired targeting of Smc4p-GFP to rDNA chromatin. Site-specific mutagenesis of the predicted protease active site cysteine and histidine residues of Smt4p abolishes the SMT4 function in vivo. The previously uncharacterized SIZ1 (SAP and Miz) gene, which encodes a protein containing a predicted DNA-binding SAP module and a Miz finger, is identified as a bypass suppressor of the growth defect associated with the SMT4 disruption. The SIZ1 gene disruption is synthetically lethal with the SIZ2 deletion. We propose that SMT4, SIZ1, and SIZ2 are involved in a novel pathway of chromosome maintenance.




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
D. C. Schwartz, R. Felberbaum, and M. Hochstrasser
The Ulp2 SUMO Protease Is Required for Cell Division following Termination of the DNA Damage Checkpoint
Mol. Cell. Biol., October 1, 2007; 27(19): 6948 - 6961.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
X. L. Chen, H. R. Silver, L. Xiong, I. Belichenko, C. Adegite, and E. S. Johnson
Topoisomerase I-Dependent Viability Loss in Saccharomyces cerevisiae Mutants Defective in Both SUMO Conjugation and DNA Repair
Genetics, September 1, 2007; 177(1): 17 - 30.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kosoy, T. M. Calonge, E. A. Outwin, and M. J. O'Connell
Fission Yeast Rnf4 Homologs Are Required for DNA Repair
J. Biol. Chem., July 13, 2007; 282(28): 20388 - 20394.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Nowak and M. Hammerschmidt
Ubc9 Regulates Mitosis and Cell Survival during Zebrafish Development
Mol. Biol. Cell, December 1, 2006; 17(12): 5324 - 5336.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Z. Wang, G. M. Jones, and G. Prelich
Genetic Analysis Connects SLX5 and SLX8 to the SUMO Pathway in Saccharomyces cerevisiae
Genetics, March 1, 2006; 172(3): 1499 - 1509.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Y. Takahashi, V. Yong-Gonzalez, Y. Kikuchi, and A. Strunnikov
SIZ1/SIZ2 Control of Chromosome Transmission Fidelity Is Mediated by the Sumoylation of Topoisomerase II
Genetics, February 1, 2006; 172(2): 783 - 794.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B.-D. Wang, D. Eyre, M. Basrai, M. Lichten, and A. Strunnikov
Condensin Binding at Distinct and Specific Chromosomal Sites in the Saccharomyces cerevisiae Genome
Mol. Cell. Biol., August 15, 2005; 25(16): 7216 - 7225.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
X. L. Chen, A. Reindle, and E. S. Johnson
Misregulation of 2{micro}m Circle Copy Number in a SUMO Pathway Mutant
Mol. Cell. Biol., May 15, 2005; 25(10): 4311 - 4320.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
F. Ayaydin and M. Dasso
Distinct In Vivo Dynamics of Vertebrate SUMO Paralogues
Mol. Biol. Cell, December 1, 2004; 15(12): 5208 - 5218.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. Roth, C. Sustmann, M. Kieslinger, A. Gilmozzi, D. Irmer, E. Kremmer, C. Turck, and R. Grosschedl
PIASy-Deficient Mice Display Modest Defects in IFN and Wnt Signaling
J. Immunol., November 15, 2004; 173(10): 6189 - 6199.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D. D'Amours and A. Amon
At the interface between signaling and executing anaphase--Cdc14 and the FEAR network
Genes & Dev., November 1, 2004; 18(21): 2581 - 2595.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Wohlschlegel, E. S. Johnson, S. I. Reed, and J. R. Yates III
Global Analysis of Protein Sumoylation in Saccharomyces cerevisiae
J. Biol. Chem., October 29, 2004; 279(44): 45662 - 45668.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Kaeberlein, A. A. Andalis, G. B. Liszt, G. R. Fink, and L. Guarente
Saccharomyces cerevisiae SSD1-V Confers Longevity by a Sir2p-Independent Mechanism
Genetics, April 1, 2004; 166(4): 1661 - 1672.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. Stead, C. Aguilar, T. Hartman, M. Drexel, P. Meluh, and V. Guacci
Pds5p regulates the maintenance of sister chromatid cohesion and is sumoylated to promote the dissolution of cohesion
J. Cell Biol., November 24, 2003; 163(4): 729 - 741.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Y. Azuma, A. Arnaoutov, and M. Dasso
SUMO-2/3 regulates topoisomerase II in mitosis
J. Cell Biol., November 10, 2003; 163(3): 477 - 487.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. R. Bylebyl, I. Belichenko, and E. S. Johnson
The SUMO Isopeptidase Ulp2 Prevents Accumulation of SUMO Chains in Yeast
J. Biol. Chem., November 7, 2003; 278(45): 44113 - 44120.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
R. de Lahondes, V. Ribes, and B. Arcangioli
Fission Yeast Sap1 Protein Is Essential for Chromosome Stability
Eukaryot. Cell, October 1, 2003; 2(5): 910 - 921.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S.-J. Li and M. Hochstrasser
The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity
J. Cell Biol., March 31, 2003; 160(7): 1069 - 1082.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Stade, F. Vogel, I. Schwienhorst, B. Meusser, C. Volkwein, B. Nentwig, R. J. Dohmen, and T. Sommer
A Lack of SUMO Conjugation Affects cNLS-dependent Nuclear Protein Import in Yeast
J. Biol. Chem., December 13, 2002; 277(51): 49554 - 49561.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. I. Tussie-Luna, B. Michel, S. Hakre, and A. L. Roy
The SUMO Ubiquitin-Protein Isopeptide Ligase Family Member Miz1/PIASxbeta /Siz2 Is a Transcriptional Cofactor for TFII-I
J. Biol. Chem., November 1, 2002; 277(45): 43185 - 43193.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. I. Tussie-Luna, D. Bayarsaihan, E. Seto, F. H. Ruddle, and A. L. Roy
Physical and functional interactions of histone deacetylase 3 with TFII-I family proteins and PIASxbeta
PNAS, October 1, 2002; 99(20): 12807 - 12812.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Mehta, X. M. Yang, C. S. Chan, M. J. Dobson, M. Jayaram, and S. Velmurugan
The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?
J. Cell Biol., August 19, 2002; 158(4): 625 - 637.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Kotaja, U. Karvonen, O. A. Janne, and J. J. Palvimo
PIAS Proteins Modulate Transcription Factors by Functioning as SUMO-1 Ligases
Mol. Cell. Biol., July 15, 2002; 22(14): 5222 - 5234.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Hang and M. Dasso
Association of the Human SUMO-1 Protease SENP2 with the Nuclear Pore
J. Biol. Chem., May 24, 2002; 277(22): 19961 - 19966.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Hirano
The ABCs of SMC proteins: two-armed ATPases for chromosome condensation, cohesion, and repair
Genes & Dev., February 15, 2002; 16(4): 399 - 414.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
P. K. Jackson
A new RING for SUMO: wrestling transcriptional responses into nuclear bodies with PIAS family E3 SUMO ligases
Genes & Dev., December 1, 2001; 15(23): 3053 - 3058.
[Full Text] [PDF]