Genetics, Vol. 158, 41-64, May 2001, Copyright © 2001

Comparative Gene Expression Profiles Following UV Exposure in Wild-Type and SOS-Deficient Escherichia coli

Justin Courcellec, Arkady Khodurskya, Brian Peterb, Patrick O. Browna, and Philip C. Hanawaltd
a Department of Biochemistry, Howard Hughes Medical Institute, Stanford University, Stanford, California 94305,
b Department of MCB, UC-Berkeley, Berkeley, California 94720,
c Department of Biological Science, Mississippi State University, Mississippi State, Mississippi 39762
d Department of Biological Sciences, Stanford University, Stanford, California 94305

Corresponding author: Justin Courcelle, Department of Biological Science, P.O. Box GY, Mississippi State University, Mississippi State, MS 39762., jcourcelle{at}biology.msstate.edu (E-mail)

Communicating editor: P. L. FOSTER

The SOS response in UV-irradiated Escherichia coli includes the upregulation of several dozen genes that are negatively regulated by the LexA repressor. Using DNA microarrays containing amplified DNA fragments from 95.5% of all open reading frames identified on the E. coli chromosome, we have examined the changes in gene expression following UV exposure in both wild-type cells and lexA1 mutants, which are unable to induce genes under LexA control. We report here the time courses of expression of the genes surrounding the 26 documented lexA-regulated regions on the E. coli chromosome. We observed 17 additional sites that responded in a lexA-dependent manner and a large number of genes that were upregulated in a lexA-independent manner although upregulation in this manner was generally not more than twofold. In addition, several transcripts were either downregulated or degraded following UV irradiation. These newly identified UV-responsive genes are discussed with respect to their possible roles in cellular recovery following exposure to UV irradiation.




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