Characterization of the S-Locus Region of Almond (Prunus dulcis): Analysis of a Somaclonal Mutant and a Cosmid Contig for an S Haplotype

Corresponding author: Hidenori Sassa, Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Maioka 641-12, Totsuka-ku, Yokohama 244-0813, Japan., sassa{at}yokohama-cu.ac.jp (E-mail)

Communicating editor: M. K. UYENOYAMA

Almond has a self-incompatibility system that is controlled by an S locus consisting of the S-RNase gene and an unidentified "pollen S gene." An almond cultivar "Jeffries," a somaclonal mutant of "Nonpareil" (S^cS^d), has a dysfunctional S^c haplotype both in pistil and pollen. Immunoblot and genomic Southern blot analyses detected no S^c haplotype-specific signal in Jeffries. Southern blot showed that Jeffries has an extra copy of the S^d haplotype. These results indicate that at least two mutations had occurred to generate Jeffries: (1) deletion of the S^c haplotype and (2) duplication of the S^d haplotype. To analyze the extent of the deletion in Jeffries and gain insight into the physical limit of the S locus region, 200 kbp of a cosmid contig for the S^c haplotype was constructed. Genomic Southern blot analyses showed that the deletion in Jeffries extends beyond the region covered by the contig. Most cosmid end probes, except those near the S^c-RNase gene, cross-hybridized with DNA fragments from different S haplotypes. This suggests that regions away from the S^c-RNase gene can recombine between different S haplotypes, implying that the cosmid contig extends to the borders of the S locus.

This article has been cited by other articles:

				J. Vieira, R. A. M. Santos, T. Habu, R. Tao, and C. P. Vieira The Prunus Self-Incompatibility Locus (S locus) Is Seldom Rearranged J. Hered., November 1, 2008; 99(6): 657 - 660. [Abstract] [Full Text] [PDF]

				J. Vieira, E. Teles, R. A. M. Santos, and C. P. Vieira Recombination at Prunus S-Locus Region SLFL1 Gene Genetics, September 1, 2008; 180(1): 483 - 491. [Abstract] [Full Text] [PDF]

				T. Tsukamoto, D. Potter, R. Tao, C. P. Vieira, J. Vieira, and A. F. Iezzoni Genetic and molecular characterization of three novel S-haplotypes in sour cherry (Prunus cerasus L.) J. Exp. Bot., August 1, 2008; 59(11): 3169 - 3185. [Abstract] [Full Text] [PDF]

				H. Sassa, H. Kakui, M. Miyamoto, Y. Suzuki, T. Hanada, K. Ushijima, M. Kusaba, H. Hirano, and T. Koba S Locus F-Box Brothers: Multiple and Pollen-Specific F-Box Genes With S Haplotype-Specific Polymorphisms in Apple and Japanese Pear Genetics, April 1, 2007; 175(4): 1869 - 1881. [Abstract] [Full Text] [PDF]

				N. R. Hauck, K. Ikeda, R. Tao, and A. F. Iezzoni The Mutated S1-Haplotype in Sour Cherry Has an Altered S-Haplotype-Specific F-Box Protein Gene J. Hered., September 1, 2006; 97(5): 514 - 520. [Abstract] [Full Text] [PDF]

				B. McClure S-RNase and SLF Determine S-Haplotype-Specific Pollen Recognition and Rejection PLANT CELL, November 1, 2004; 16(11): 2840 - 2847. [Full Text] [PDF]

				T.-h. Kao and T. Tsukamoto The Molecular and Genetic Bases of S-RNase-Based Self-Incompatibility PLANT CELL, June 1, 2004; 16(suppl_1): S72 - S83. [Full Text] [PDF]

				H. Qiao, H. Wang, L. Zhao, J. Zhou, J. Huang, Y. Zhang, and Y. Xue The F-Box Protein AhSLF-S2 Physically Interacts with S-RNases That May Be Inhibited by the Ubiquitin/26S Proteasome Pathway of Protein Degradation during Compatible Pollination in Antirrhinum PLANT CELL, March 1, 2004; 16(3): 582 - 595. [Abstract] [Full Text] [PDF]

				H. Yamane, K. Ikeda, K. Ushijima, H. Sassa, and R. Tao A Pollen-Expressed Gene for a Novel Protein with an F-box Motif that is Very Tightly Linked to a Gene for S-RNase in Two Species of Cherry, Prunus cerasus and P. avium Plant Cell Physiol., July 15, 2003; 44(7): 764 - 769. [Abstract] [Full Text] [PDF]

				K. Ushijima, H. Sassa, A. M. Dandekar, T. M. Gradziel, R. Tao, and H. Hirano Structural and Transcriptional Analysis of the Self-Incompatibility Locus of Almond: Identification of a Pollen-Expressed F-Box Gene with Haplotype-Specific Polymorphism PLANT CELL, March 1, 2003; 15(3): 771 - 781. [Abstract] [Full Text] [PDF]

				Y. Wang, X. Wang, A. L. Skirpan, and T.-h. Kao S-RNase-mediated self-incompatibility J. Exp. Bot., January 1, 2003; 54(380): 115 - 122. [Abstract] [Full Text] [PDF]

				J. F. Golz, H.-Y. Oh, V. Su, M. Kusaba, and E. Newbigin Genetic analysis of Nicotiana pollen-part mutants is consistent with the presence of an S-ribonuclease inhibitor at the S locus PNAS, December 18, 2001; 98(26): 15372 - 15376. [Abstract] [Full Text] [PDF]