Genetics, Vol. 157, 1307-1312, March 2001, Copyright © 2001

A Targeted Gene Knockout in Drosophila

Yikang S. Ronga and Kent G. Golica
a Department of Biology, University of Utah, Salt Lake City, Utah 84112

Corresponding author: Kent G. Golic, Department of Biology, University of Utah, 257 S. 1400 East, 201 Biology Bldg., Salt Lake City, UT 84112., golic{at}bioscience.utah.edu (E-mail)

Communicating editor: R. S. HAWLEY

We previously described a method for targeted homologous recombination at the yellow gene of Drosophila melanogaster. Because only a single gene was targeted, further work was required to show whether the method could be extended to become generally useful for gene modification in Drosophila. We have now used this method to produce a knockout of the autosomal pugilist gene by homologous recombination between the endogenous locus and a 2.5-kb DNA fragment. This was accomplished solely by tracking the altered genetic linkage of an arbitrary marker gene as the targeting DNA moved from chromosome X or 2 to chromosome 3. The results indicate that this method of homologous recombination is likely to be generally useful for Drosophila gene targeting.




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