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Genetics, Vol. 157, 1277-1284, March 2001, Copyright © 2001

An Amplified Fragment Length Polymorphism Map of the Silkworm

Yuan-De Tana,b, Chunling Wanb, Yufang Zhub, Chen Luc, Zhonghuai Xiangc, and Hong-Wen Denga,d
a Laboratory of Molecular and Statistical Genetics, College of Life Science, Hunan Normal University, Changsha 410081, People's Republic of China,
b College of Life Science, Nangjing Normal University, Nanjing 210097, People's Republic of China,
c Key Laboratory of Agricultural Ministry, College of Sericulture, Southwest Agricultural University, Chongqing 400716, People's Republic of China
d Osteoporosis Research Center and Department of Biomedical Sciences, Creighton University, Omaha, Nebraska 68131

Corresponding author: Hong-Wen Deng, Osteoporosis Research Ctr., Creighton University, 601 N. 30th St., Suite 6787, Omaha, NE 68131., deng{at}creighton.edu (E-mail)

Communicating editor: M. J. SIMMONS

The silkworm (Bombyx mori L.) is a lepidopteran insect with a long history of significant agricultural value. We have constructed the first amplified fragment length polymorphism (AFLP) genetic linkage map of the silkworm B. mori at a LOD score of 2.5. The mapping AFLP markers were genotyped in 47 progeny from a backcross population of the cross no. 782 x od100. A total of 1248 (60.7%) polymorphic AFLP markers were detected with 35 PstI/TaqI primer combinations. Each of the primer combinations generated an average of 35.7 polymorphic AFLP markers. A total of 545 (44%) polymorphic markers are consistent with the expected segregation ratio of 1:1 at the significance level of P = 0.05. Of the 545 polymorphic markers, 356 were assigned to 30 linkage groups. The number of markers on linkage groups ranged from 4 to 36. There were 21 major linkage groups with 7–36 markers and 9 relatively small linkage groups with 4–6 markers. The 30 linkage groups varied in length from 37.4 to 691.0 cM. The total length of this AFLP linkage map was 6512 cM. Genetic distances between two neighboring markers on the same linkage group ranged from 0.2 to 47 cM with an average of 18.2 cM. The sex-linked gene od was located between the markers P1T3B40 and P3T3B27 at the end of group 3, indicating that AFLP linkage group 3 was the Z (sex) chromosome. This work provides an essential basic map for constructing a denser linkage map and for mapping genes underlying agronomically important traits in the silkworm B. mori L.




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