A Novel Method for Estimating Substitution Rate Variation Among Sites in a Large Dataset of Homologous DNA Sequences

Corresponding author: Graziano Pesole, Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, via Celoria 26, 20133 Milano, Italy., graziano.pesole{at}unimi.it (E-mail)

Communicating editor: F. TAJIMA

We present here a novel method to estimate the site-specific relative variability in large sets of homologous sequences. It is based on the simple idea that the more closely related are the compared sequences, the higher the probability of observing nucleotide changes at rapidly evolving sites. A simulation study has been carried out to support the reliability of the method, which has been applied also to analyzing the site variability of all available human sequences corresponding to the two hypervariable regions of the mitochondrial D-loop.

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