Toward a Physical Map of Drosophila buzzatii: Use of Randomly Amplified Polymorphic DNA Polymorphisms and Sequence-Tagged Site Landmarks

Corresponding author: Mauro Santos, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Facultat de Ciències, Edifici Cn, 08193 Bellaterra (Barcelona), Spain., m.santos{at}cc.uab.es (E-mail)

Communicating editor: L. PARTRIDGE

We present a physical map based on RAPD polymorphic fragments and sequence-tagged sites (STSs) for the repleta group species Drosophila buzzatii. One hundred forty-four RAPD markers have been used as probes for in situ hybridization to the polytene chromosomes, and positive results allowing the precise localization of 108 RAPDs were obtained. Of these, 73 behave as effectively unique markers for physical map construction, and in 9 additional cases the probes gave two hybridization signals, each on a different chromosome. Most markers (68%) are located on chromosomes 2 and 4, which partially agree with previous estimates on the distribution of genetic variation over chromosomes. One RAPD maps close to the proximal breakpoint of inversion 2z³ but is not included within the inverted fragment. However, it was possible to conclude from this RAPD that the distal breakpoint of 2z³ had previously been wrongly assigned. A total of 39 cytologically mapped RAPDs were converted to STSs and yielded an aggregate sequence of 28,431 bp. Thirty-six RAPDs (25%) did not produce any detectable hybridization signal, and we obtained the DNA sequence from three of them. Further prospects toward obtaining a more developed genetic map than the one currently available for D. buzzatii are discussed.

This article has been cited by other articles:

				J. Gonzalez, M. Nefedov, I. Bosdet, F. Casals, O. Calvete, A. Delprat, H. Shin, R. Chiu, C. Mathewson, N. Wye, et al. A BAC-based physical map of the Drosophila buzzatii genome Genome Res., June 1, 2005; 15(6): 885 - 892. [Abstract] [Full Text] [PDF]

				R. Piccinali, M. Aguade, and E. Hasson Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae Mol. Biol. Evol., January 1, 2004; 21(1): 141 - 152. [Abstract] [Full Text] [PDF]

				H. Laayouni, E. Hasson, M. Santos, and A. Fontdevila The Evolutionary History of Drosophila buzzatii. XXXV. Inversion Polymorphism and Nucleotide Variability in Different Regions of the Second Chromosome Mol. Biol. Evol., June 1, 2003; 20(6): 931 - 944. [Abstract] [Full Text] [PDF]

				G. A. Gomez and E. Hasson Transpecific Polymorphisms in an Inversion Linked Esterase Locus in Drosophila buzzatii Mol. Biol. Evol., March 1, 2003; 20(3): 410 - 423. [Abstract] [Full Text] [PDF]

				J. Gonzalez, J. M. Ranz, and A. Ruiz Chromosomal Elements Evolve at Different Rates in the Drosophila Genome Genetics, July 1, 2002; 161(3): 1137 - 1154. [Abstract] [Full Text] [PDF]