Specific Genetic Interference With Behavioral Rhythms in Drosophila by Expression of Inverted Repeats

Specific Genetic Interference With Behavioral Rhythms in Drosophila by Expression of Inverted Repeats

Sebastian Martinek^a and Michael W. Young^a
^a Laboratory of Genetics, and National Science Foundation Science and Technology Center for Biological Timing, The Rockefeller University, New York, New York 10021

Corresponding author: Michael W. Young, Laboratory of Genetics, The Rockefeller University, 1230 York Ave., New York, NY 10021., young{at}rockvax.rockefeller.edu (E-mail)

Communicating editor: J. J. LOROS

We describe a new experimental technique that allows for a tissue-specificreduction of gene activity in the Drosophila nervous system.On the basis of the observation that certain gene functionscan be ubiquitously blocked by injecting double-stranded RNAinto Drosophila embryos, we employed a method to interfere withan individual gene function permanently in a predetermined celltype. This was achieved by the formation of an inverted-repeatRNA sequence in the tissue of interest under control of theGAL4/UAS binary expression system. As an example, we show thatinverted-repeat-mediated interference with the period gene producesa hypomorphic period phenotype. A selective decrease of periodRNA appears to be a component of the cellular response.

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