Synthetic Interactions of the Post-Golgi sec Mutations of Saccharomyces cerevisiae

Corresponding author: Peter Novick, Department of Cell Biology, Yale University School of Medicine, P.O. Box 208002, New Haven, CT 06520-8002., peter.novick{at}yale.edu (E-mail)

Communicating editor: D. BOTSTEIN

In the budding yeast Saccharomyces cerevisiae, synthetic lethality has been extensively used both to characterize interactions between genes previously identified as likely to be involved in similar processes as well as to uncover new interactions. We have performed a large study of the synthetic lethal interactions of the post-Golgi sec mutations. Included in this study are the interactions of the post-Golgi sec mutations with each other, with mutations affecting earlier stages of the secretory pathway, with selected mutations affecting the actin cytoskeleton, and with selected cell division cycle (cdc) mutations affecting processes thought to be important for or involving secretion, such as polarity establishment and cytokinesis. Synthetic negative interactions of the post-Golgi sec mutations appear (as predicted) to be largely stage specific, although there are some notable exceptions. The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation.

This article has been cited by other articles:

				J. A. Songer and M. Munson Sec6p Anchors the Assembled Exocyst Complex at Sites of Secretion Mol. Biol. Cell, February 1, 2009; 20(3): 973 - 982. [Abstract] [Full Text] [PDF]

				C. Kurischko, V. K. Kuravi, N. Wannissorn, P. A. Nazarov, M. Husain, C. Zhang, K. M. Shokat, J. M. McCaffery, and F. C. Luca The Yeast LATS/Ndr Kinase Cbk1 Regulates Growth via Golgi-dependent Glycosylation and Secretion Mol. Biol. Cell, December 1, 2008; 19(12): 5559 - 5578. [Abstract] [Full Text] [PDF]

				Y. Fang, R. Sugiura, Y. Ma, T. Yada-Matsushima, H. Umeno, and T. Kuno Cation Diffusion Facilitator Cis4 Is Implicated in Golgi Membrane Trafficking via Regulating Zinc Homeostasis in Fission Yeast Mol. Biol. Cell, April 1, 2008; 19(4): 1295 - 1303. [Abstract] [Full Text] [PDF]

				Y. Ma, T. Kuno, A. Kita, T. Nabata, S. Uno, and R. Sugiura Genetic Evidence for Phospholipid-Mediated Regulation of the Rab GDP-Dissociation Inhibitor in Fission Yeast Genetics, November 1, 2006; 174(3): 1259 - 1271. [Abstract] [Full Text] [PDF]

				E. Milgrom, R. W. West Jr., C. Gao, and W.-C. W. Shen TFIID and Spt-Ada-Gcn5-Acetyltransferase Functions Probed by Genome-wide Synthetic Genetic Array Analysis Using a Saccharomyces cerevisiae taf9-ts Allele Genetics, November 1, 2005; 171(3): 959 - 973. [Abstract] [Full Text] [PDF]

				L. F. Ciufo, J. W. Barclay, R. D. Burgoyne, and A. Morgan Munc18-1 Regulates Early and Late Stages of Exocytosis via Syntaxin-independent Protein Interactions Mol. Biol. Cell, February 1, 2005; 16(2): 470 - 482. [Abstract] [Full Text] [PDF]

				C. Z. Chen, M. Calero, C. J. DeRegis, M. Heidtman, C. Barlowe, and R. N. Collins Genetic Analysis of Yeast Yip1p Function Reveals a Requirement for Golgi-Localized Rab Proteins and Rab-Guanine Nucleotide Dissociation Inhibitor Genetics, December 1, 2004; 168(4): 1827 - 1841. [Abstract] [Full Text] [PDF]

				O. C. Rodriguez and R. E. Cheney Human myosin-Vc is a novel class V myosin expressed in epithelial cells J. Cell Sci., January 3, 2002; 115(5): 991 - 1004. [Abstract] [Full Text] [PDF]

				X. Zhang, E. Bi, P. Novick, L. Du, K. G. Kozminski, J. H. Lipschutz, and W. Guo Cdc42 Interacts with the Exocyst and Regulates Polarized Secretion J. Biol. Chem., December 7, 2001; 276(50): 46745 - 46750. [Abstract] [Full Text] [PDF]

				J. E. Adamo, J. J. Moskow, A. S. Gladfelter, D. Viterbo, D. J. Lew, and P. J. Brennwald Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud J. Cell Biol., November 12, 2001; 155(4): 581 - 592. [Abstract] [Full Text] [PDF]