A Screen for Dominant Modifiers of ro^Dom, a Mutation That Disrupts Morphogenetic Furrow Progression in Drosophila, Identifies Groucho and Hairless as Regulators of atonal Expression

Corresponding author: Françoise Chanut, Department of Anatomy, S-1334, Box 0452, University of California, 513 Parnassus Ave., San Francisco, CA 94143., chanut{at}itsa.ucsf.edu (E-mail)

Communicating editor: T. SCHÜPBACH

ro^Dom is a dominant allele of rough (ro) that results in reduced eye size due to premature arrest in morphogenetic furrow (MF) progression. We found that the ro^Dom stop-furrow phenotype was sensitive to the dosage of genes known to affect retinal differentiation, in particular members of the hedgehog (hh) signaling cascade. We demonstrate that ro^Dom interferes with Hh's ability to induce the retina-specific proneural gene atonal (ato) in the MF and that normal eye size can be restored by providing excess Ato protein. We used ro^Dom as a sensitive genetic background in which to identify mutations that affect hh signal transduction or regulation of ato expression. In addition to mutations in several unknown loci, we recovered multiple alleles of groucho (gro) and Hairless (H). Analysis of their phenotypes in somatic clones suggests that both normally act to restrict neuronal cell fate in the retina, although they control different aspects of ato's complex expression pattern.

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