A Screen for Dominant Modifiers of the irreC-rst Cell Death Phenotype in the Developing Drosophila Retina

A Screen for Dominant Modifiers of the irreC-rst Cell Death Phenotype in the Developing Drosophila Retina

Sara B. Tanenbaum^a, Sharon M. Gorski^a, Jamie C. Rusconi^a, and Ross L. Cagan^a
^a Department of Molecular Biology and Pharmacology, Washington University School of Medicine, Saint Louis, Missouri 63110

Corresponding author: Ross L. Cagan, Department of Molecular Biology and Pharmacology, Washington University School of Medicine, Campus Box 8103, 660 S. Euclid Ave., St. Louis, MO 63110., cagan{at}molecool.wustl.edu (E-mail)

Communicating editor: K. ANDERSON

Programmed cell death (PCD) in the Drosophila retina requiresactivity of the irregular chiasmC-roughest (irreC-rst) gene.Loss-of-function mutations in irreC-rst block PCD during retinaldevelopment and lead to a rough eye phenotype in the adult.To identify genes that interact with irreC-rst and may be involvedin PCD, we conducted a genetic screen for dominant enhancersand suppressors of the adult rough eye phenotype. We screened150,000 mutagenized flies and recovered 170 dominant modifiersthat localized primarily to the second and third chromosomes.At least two allelic groups correspond to previously identifieddeath regulators, Delta and dRas1. Examination of retinae fromhomozygous viable mutants indicated two major phenotypic classes.One class exhibited pleiotropic defects while the other classexhibited defects specific to the cell population that normallyundergoes PCD.

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