Pep3p/Pep5p Complex: A Putative Docking Factor at Multiple Steps of Vesicular Transport to the Vacuole of Saccharomyces cerevisiae

Corresponding author: Carol A. Woolford, Carnegie Mellon University, Department of Biological Sciences, Mellon Institute, 4400 Fifth Ave., Pittsburgh, PA 15213., cw2g{at}andrew.cmu.edu (E-mail)

Communicating editor: A. P. MITCHELL

Pep3p and Pep5p are known to be necessary for trafficking of hydrolase precursors to the vacuole and for vacuolar biogenesis. These proteins are present in a hetero-oligomeric complex that mediates transport at the vacuolar membrane. PEP5 interacts genetically with VPS8, implicating Pep5p in the earlier Golgi to endosome step and/or in recycling from the endosome to the Golgi. To understand further the cellular roles of Pep3p and Pep5p, we isolated and characterized a set of pep3 conditional mutants. Characterization of mutants revealed that pep3^ts mutants are defective in the endosomal and nonendosomal Golgi to vacuole transport pathways, in the cytoplasm to vacuole targeting pathway, in recycling from the endosome back to the late Golgi, and in endocytosis. PEP3 interacts genetically with two members of the endosomal SNARE complex, PEP12 (t-SNARE) and PEP7 (homologue of mammalian EEA1); Pep3p and Pep5p associate physically with Pep7p as revealed by two-hybrid analysis. Our results suggest that a core Pep3p/Pep5p complex promotes vesicular docking/fusion reactions in conjunction with SNARE proteins at multiple steps in transport routes to the vacuole. We propose that this complex may be responsible for tethering transport vesicles on target membranes.

This article has been cited by other articles:

				G.-d. Zhu, G. Salazar, S. A. Zlatic, B. Fiza, M. M. Doucette, C. J. Heilman, A. I. Levey, V. Faundez, and S. W. L'Hernault SPE-39 Family Proteins Interact with the HOPS Complex and Function in Lysosomal Delivery Mol. Biol. Cell, February 1, 2009; 20(4): 1223 - 1240. [Abstract] [Full Text] [PDF]

				R. Puria, S. A. Zurita-Martinez, and M. E. Cardenas From the Cover: Nuclear translocation of Gln3 in response to nutrient signals requires Golgi-to-endosome trafficking in Saccharomyces cerevisiae PNAS, May 20, 2008; 105(20): 7194 - 7199. [Abstract] [Full Text] [PDF]

				S. A. Zurita-Martinez, R. Puria, X. Pan, J. D. Boeke, and M. E. Cardenas Efficient Tor Signaling Requires a Functional Class C Vps Protein Complex in Saccharomyces cerevisiae Genetics, August 1, 2007; 176(4): 2139 - 2150. [Abstract] [Full Text] [PDF]

				K. Franke, M. Nguyen, A. Hartl, H.-M. Dahse, G. Vogl, R. Wurzner, P. F. Zipfel, W. Kunkel, and R. Eck The vesicle transport protein Vac1p is required for virulence of Candida albicans. Microbiology, October 1, 2006; 152(Pt 10): 3111 - 3121. [Abstract] [Full Text] [PDF]

				G. Wang and R. J. Deschenes Plasma Membrane Localization of Ras Requires Class C Vps Proteins and Functional Mitochondria in Saccharomyces cerevisiae Mol. Cell. Biol., April 15, 2006; 26(8): 3243 - 3255. [Abstract] [Full Text] [PDF]

				G. E. Palmer, M. N. Kelly, and J. E. Sturtevant The Candida albicans Vacuole Is Required for Differentiation and Efficient Macrophage Killing Eukaryot. Cell, October 1, 2005; 4(10): 1677 - 1686. [Abstract] [Full Text] [PDF]

				S. Pulipparacharuvil, M. A. Akbar, S. Ray, E. A. Sevrioukov, A. S. Haberman, J. Rohrer, and H. Kramer Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules J. Cell Sci., August 15, 2005; 118(16): 3663 - 3673. [Abstract] [Full Text] [PDF]

				K. C. Sadler, A. Amsterdam, C. Soroka, J. Boyer, and N. Hopkins A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver disease Development, August 1, 2005; 132(15): 3561 - 3572. [Abstract] [Full Text] [PDF]

				T. J. LaGrassa and C. Ungermann The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complex J. Cell Biol., January 31, 2005; 168(3): 401 - 414. [Abstract] [Full Text] [PDF]

				S. Subramanian, C. A. Woolford, and E. W. Jones The Sec1/Munc18 Protein, Vps33p, Functions at the Endosome and the Vacuole of Saccharomyces cerevisiae Mol. Biol. Cell, June 1, 2004; 15(6): 2593 - 2605. [Abstract] [Full Text] [PDF]

				C. M. Coughlan, J. L. Walker, J. C. Cochran, K. D. Wittrup, and J. L. Brodsky Degradation of Mutated Bovine Pancreatic Trypsin Inhibitor in the Yeast Vacuole Suggests Post-endoplasmic Reticulum Protein Quality Control J. Biol. Chem., April 9, 2004; 279(15): 15289 - 15297. [Abstract] [Full Text] [PDF]

				S. C. W. Richardson, S. C. Winistorfer, V. Poupon, J. P. Luzio, and R. C. Piper Mammalian Late Vacuole Protein Sorting Orthologues Participate in Early Endosomal Fusion and Interact with the Cytoskeleton Mol. Biol. Cell, March 1, 2004; 15(3): 1197 - 1210. [Abstract] [Full Text] [PDF]

				V. Poupon, A. Stewart, S. R. Gray, R. C. Piper, and J. P. Luzio The Role of mVps18p in Clustering, Fusion, and Intracellular Localization of Late Endocytic Organelles Mol. Biol. Cell, October 1, 2003; 14(10): 4015 - 4027. [Abstract] [Full Text] [PDF]

				G. E. Palmer, A. Cashmore, and J. Sturtevant Candida albicans VPS11 Is Required for Vacuole Biogenesis and Germ Tube Formation Eukaryot. Cell, June 1, 2003; 2(3): 411 - 421. [Abstract] [Full Text] [PDF]

				A. J. Koning, L. L. Larson, E. J. Cadera, M. L. Parrish, and R. L. Wright Mutations That Affect Vacuole Biogenesis Inhibit Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae Genetics, April 1, 2002; 160(4): 1335 - 1352. [Abstract] [Full Text] [PDF]

				J. R. C. Whyte and S. Munro Vesicle tethering complexes in membrane traffic J. Cell Sci., January 7, 2002; 115(13): 2627 - 2637. [Abstract] [Full Text] [PDF]