Distinct P-Element Excision Products in Somatic and Germline Cells of Drosophila melanogaster

Corresponding author: Gregory B. Gloor, Department of Biochemistry, Faculty of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 5C1, Canada., ggloor{at}julian.uwo.ca (E-mail)

Communicating editor: M. J. SIMMONS

The footprints remaining following somatic P-element excision from the Drosophila white locus were recovered and characterized. Two different types of footprints were observed. Over 75% of the footprints were short, composed of 4 or 7 nucleotides of the P-element inverted terminal repeat, and were similar to those found in a previously described plasmid excision assay. The remaining footprints were composed of 14–18 nucleotides of both inverted terminal repeats. These large footprints were indistinguishable from those recovered following germline P-element excision. Enhanced expression of the Drosophila homologue of the Ku70 protein did not affect the structure of the somatic footprints. Therefore, this protein is not a limiting factor for double-strand break repair by nonhomologous end-joining in Drosophila somatic cells.

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