help button home button Genetics Appl Env Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tomso, D. J.
Right arrow Articles by Kreuzer, K. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tomso, D. J.
Right arrow Articles by Kreuzer, K. N.
Genetics, Vol. 155, 1493-1504, August 2000, Copyright © 2000

Double-Strand Break Repair in Tandem Repeats During Bacteriophage T4 Infection

Daniel J. Tomsoa and Kenneth N. Kreuzera
a Duke University Medical Center, Durham, North Carolina 27710

Corresponding author: Kenneth N. Kreuzer, Box 3020, Duke University Medical Center, Durham, NC 27710., kenneth.kreuzer{at}duke.edu (E-mail)

Communicating editor: R. MAURER

Recombinational repair of double-strand breaks in tandemly repeated sequences often results in the loss of one or more copies of the repeat. The single-strand annealing (SSA) model for repair has been proposed to account for this nonconservative recombination. In this study we present a plasmid-based physical assay that measures SSA during bacteriophage T4 infection and apply this assay to the genetic analysis of break repair. SSA occurs readily in broken plasmid DNA and is independent of the strand exchange protein UvsX and its accessory factor UvsY. We use the unique features of T4 DNA metabolism to examine the link between SSA repair and DNA replication and demonstrate directly that the DNA polymerase and the major replicative helicase of the phage are not required for SSA repair. We also show that the Escherichia coli RecBCD enzyme can mediate the degradation of broken DNA during early, but not late, times of infection. Finally, we consider the status of broken ends during the course of the infection and propose a model for SSA during T4 infections.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. C. Dudas and K. N. Kreuzer
Bacteriophage T4 Helicase Loader Protein gp59 Functions as Gatekeeper in Origin-dependent Replication in Vivo
J. Biol. Chem., June 3, 2005; 280(22): 21561 - 21569.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
E. S. Miller, E. Kutter, G. Mosig, F. Arisaka, T. Kunisawa, and W. Ruger
Bacteriophage T4 Genome
Microbiol. Mol. Biol. Rev., March 1, 2003; 67(1): 86 - 156.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. R. Preston, W. Engels, and C. Flores
Efficient Repair of DNA Breaks in Drosophila: Evidence for Single-Strand Annealing and Competition With Other Repair Pathways
Genetics, June 1, 2002; 161(2): 711 - 720.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
X.-D. Yao and D. H. Evans
Effects of DNA Structure and Homology Length on Vaccinia Virus Recombination
J. Virol., August 1, 2001; 75(15): 6923 - 6932.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. W. George, B. A. Stohr, D. J. Tomso, and K. N. Kreuzer
The tight linkage between DNA replication and double-strand break repair in bacteriophage T4
PNAS, July 17, 2001; 98(15): 8290 - 8297.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Bzymek and S. T. Lovett
Evidence for Two Mechanisms of Palindrome-Stimulated Deletion in Escherichia coli: Single-Strand Annealing and Replication Slipped Mispairing
Genetics, June 1, 2001; 158(2): 527 - 540.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Genetics Society of America.