Genetics, Vol. 155, 1331-1345, July 2000, Copyright © 2000

A Microsatellite Linkage Map of Rainbow Trout (Oncorhynchus mykiss) Characterized by Large Sex-Specific Differences in Recombination Rates

Takashi Sakamotoa,c, Roy G. Danzmanna, Karim Gharbib, Pamela Howarda, Akiyuki Ozakic, Sok Kean Khooc, Rachael A. Worama, Nobuaki Okamotoc, Moira M. Fergusona, Lars-Erik Holmd, René Guyomardb, and Bjorn Hoyheime
a Department of Zoology, University of Guelph, Guelph, Ontario N1G 2W1, Canada,
b Laboratoire de Génétique des Poissons, Institut National de la Recherche Agronomique, Jouy-en-Josas France, 78352,
c Department of Aquatic Biosciences, Tokyo University of Fisheries, Minato, Tokyo 108, Japan,
d Department of Animal Breeding and Genetics, Danish Institute of Agricultural Science, DK-8830, Tjele, Denmark
e Department of Morphology, Genetics and Aquatic Biology, Norwegian School of Veterinary Science, N-0033, Oslo, Norway

Corresponding author: Roy G. Danzmann, Department of Zoology, University of Guelph, Guelph, Ontario N1G 2W1, Canada., rdanzman{at}uoguelph.ca (E-mail)

Communicating editor: G. A. CHURCHILL

We constructed a genetic linkage map for a tetraploid derivative species, the rainbow trout (Oncorhynchus mykiss), using 191 microsatellite, 3 RAPD, 7 ESMP, and 7 allozyme markers in three backcross families. The linkage map consists of 29 linkage groups with potential arm displacements in the female map due to male-specific pseudolinkage arrangements. Synteny of duplicated microsatellite markers was used to identify and confirm some previously reported pseudolinkage arrangements based upon allozyme markers. Fifteen centromeric regions (20 chromosome arms) were identified with a half-tetrad analysis using gynogenetic diploids. Female map length is ~10 M, but this is a large underestimate as many genotyped segments remain unassigned at a LOD threshold of 3.0. Extreme differences in female:male map distances were observed (ratio F:M, 3.25:1). Females had much lower recombination rates (0.14:1) in telomeric regions than males, while recombination rates were much higher in females within regions proximal to the centromere (F:M, 10:1). Quadrivalent formations that appear almost exclusively in males are postulated to account for the observed differences.




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