Abnormal Turning Behavior in Drosophila Larvae: Identification and Molecular Analysis of scribbler (sbb)

Abnormal Turning Behavior in Drosophila Larvae: Identification and Molecular Analysis of scribbler (sbb)

Ping Yang^a,b, Susan A. Shaver^a,b, Arthur J. Hilliker^b, and Marla B. Sokolowski^a
^a Department of Zoology, University of Toronto, Mississauga, Ontario L5L 1C6, Canada
^b Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada

Corresponding author: Marla B. Sokolowski, Department of Zoology, University of Toronto at Mississauga, Mississauga, Ontario L5L 1C6, Canada., msokolow{at}credit.erin.utoronto.ca (E-mail)

Communicating editor: T. F. C. MACKAY

Our genetic dissection of behavior has isolated scribbler (sbb),a vital gene that encodes a novel protein expressed in the embryonicand larval nervous systems and in the imaginal discs. Larvaewith mutations in sbb exhibit abnormally high amounts of turningbehavior in the absence of food. sbb is a large gene spanning>50 kb of genomic DNA with four major developmentally regulatedtranscripts. Transgenic rescue of scribbler behavior was demonstratedby targeting expression of a normal sbb transgene (sbb⁺) expressingone of the major transcripts to the nervous system. The vitalfunction of sbb was restored by ubiquitous expression of thistransgene throughout development.

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