Narrowing the Critical Regions for Mouse t Complex Transmission Ratio Distortion Factors by Use of Deletions

Corresponding author: Mary F. Lyon, MRC Mammalian Genetics Unit, Harwell, Didcot, Oxon OX11 0RD, United Kingdom., m.lyon{at}har.mrc.ac.uk (E-mail)

Communicating editor: N. A. JENKINS

Previously a deletion in mouse chromosome 17, T^22H, was shown to behave like a t allele of the t complex distorter gene Tcd1, and this was attributed to deletion of this locus. Seven further deletions are studied here, with the aim of narrowing the critical region in which Tcd1 must lie. One deletion, T^30H, together with three others, T^31H, T^33H, and T^36H, which extended more proximally, caused male sterility when heterozygous with a complete t haplotype and also enhanced transmission ratio of the partial t haplotype t⁶, and this was attributed to deletion of the Tcd1 locus. The deletions T^29H, T^32H, and T^34H that extended less proximally than T^30H permitted male fertility when opposite a complete t haplotype. These results enabled narrowing of the critical interval for Tcd1 to between the markers D17Mit164 and D17Leh48. In addition, T^29H and T^32H enhanced the transmission ratio of t⁶, but significantly less so than T^30H. T^34H had no effect on transmission ratio. These results could be explained by a new distorter located between the breakpoints of T^29H and T^34H (between T and D17Leh66E). It is suggested that the original distorter Tcd1 in fact consists of two loci: Tcd1a, lying between D17Mit164 and D17Leh48, and Tcd1b, lying between T and D17Leh66E.

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