help button home button Genetics AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Casavant, N. C.
Right arrow Articles by Wichman, H. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Casavant, N. C.
Right arrow Articles by Wichman, H. A.
Genetics, Vol. 154, 1809-1817, April 2000, Copyright © 2000

The End of the LINE?: Lack of Recent L1 Activity in a Group of South American Rodents

N. Carol Casavanta, LuAnn Scotta, Michael A. Cantrella, Lara E. Wigginsb, Robert J. Bakerb, and Holly A. Wichmana
a Department of Biological Sciences, University of Idaho, Moscow, Idaho 83844
b Department of Biological Sciences and the Museum, Texas Tech University, Lubbock, Texas 79409

Corresponding author: Holly A. Wichman, Department of Biological Sciences, University of Idaho, Moscow, ID 83844-3051., hwichman{at}uidaho.edu (E-mail)

Communicating editor: W. F. EANES

L1s (LINE-1: Long Interspersed Nuclear Element 1) are present in all mammals examined to date. They occur in both placental mammals and marsupials and thus are thought to have been present in the genome prior to the mammalian radiation. This unusual conservation of a transposable element family for over 100 million years has led to speculation that these elements provide an advantage to the genomes they inhabit. We have recently identified a group of South American rodents, including rice rats (Oryzomys), in which L1s appear to be quiescent or extinct. Several observations support this conclusion. First, genomic Southern blot analysis fails to reveal genus-specific bands in Oryzomys. Second, we were unable to find recently inserted elements. Procedures to enrich for young elements did not yield any with an intact open reading frame for reverse transcriptase; all elements isolated had numerous insertions, deletions, and stop codons. Phylogenetic analysis failed to yield species-specific clusters among the L1 elements isolated, and all Oryzomys sequences had numerous private mutations. Finally, in situ hybridization of L1 to Oryzomys chromosomes failed to reveal the characteristic L1 distribution in Oryzomys with either a homologous or heterologous probe. Thus, Oryzomys is a viable candidate for L1 extinction from a mammalian host.





This article has been cited by other articles:


Home page
Genome Res.Home page
V. P. Belancio, D. J. Hedges, and P. Deininger
Mammalian non-LTR retrotransposons: For better or worse, in sickness and in health
Genome Res., March 1, 2008; 18(3): 343 - 358.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
M. A. Cantrell, L. Scott, C. J. Brown, A. R. Martinez, and H. A. Wichman
Loss of LINE-1 Activity in the Megabats
Genetics, January 1, 2008; 178(1): 393 - 404.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
M. A. Cantrell, M. M. Ederer, I. K. Erickson, V. J. Swier, R. J. Baker, and H. A. Wichman
MysTR: an Endogenous Retrovirus Family in Mammals That Is Undergoing Recent Amplifications to Unprecedented Copy Numbers
J. Virol., December 1, 2005; 79(23): 14698 - 14707.
[Abstract] [Full Text] [PDF]


Home page
Mol Biol EvolHome page
C. Feschotte
Merlin, a New Superfamily of DNA Transposons Identified in Diverse Animal Genomes and Related to Bacterial IS1016 Insertion Sequences
Mol. Biol. Evol., September 1, 2004; 21(9): 1769 - 1780.
[Abstract] [Full Text] [PDF]


Home page
Genome Res.Home page
A. Toyoda, H. Noguchi, T. D. Taylor, T. Ito, M. T. Pletcher, Y. Sakaki, R. H. Reeves, and M. Hattori
Comparative Genomic Sequence Analysis of the Human Chromosome 21 Down Syndrome Critical Region
Genome Res., September 1, 2002; 12(9): 1323 - 1332.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
I. Arkhipova and M. Meselson
Transposable elements in sexual and ancient asexual taxa
PNAS, December 19, 2000; 97(26): 14473 - 14477.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
P. Yang, S. A. Shaver, A. J. Hilliker, and M. B. Sokolowski
Abnormal Turning Behavior in Drosophila Larvae: Identification and Molecular Analysis of scribbler (sbb)
Genetics, July 1, 2000; 155(3): 1161 - 1174.
[Abstract] [Full Text]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Genetics Society of America.