Genetics, Vol. 154, 1773-1784, April 2000, Copyright © 2000

A Detailed Linkage Map of Medaka, Oryzias latipes: Comparative Genomics and Genome Evolution

Kiyoshi Narusea, Shoji Fukamachia, Hiroshi Mitania,b, Mariko Kondoa, Tomoko Matsuokaa, Shu Kondoa, Nana Hanamuraa, Yutaka Moritaa, Kazuhito Hasegawaa, Reiko Nishigakia, Atsuko Shimadaa, Hironori Wadac, Takehiro Kusakabed, Norio Suzukid, Masato Kinoshitae, Akira Kanamorif, Tokio Teradog, Hiroshi Kimurag, Masaru Nonakaa, and Akihiro Shimaa,b
a Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan,
b Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan,
c Laboratory of Developmental Gene Regulation, Brain Science Institutes, RIKEN, Wako-shi, Saitama 351-0198, Japan,
d Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan,
e Department of Fisheries, Faculty of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan,
f National Research Institute of Aquaculture, Tamaki, Mie 519-0423, Japan
g Department of Experimental Radiology, Shiga University of Medical Science, Ohtsu-shi, Shiga 520-2192, Japan

Corresponding author: Kiyoshi Naruse, Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku 7-3-1, Tokyo 113-0033, Japan., naruse{at}biol.s.u-tokyo.ac.jp (E-mail)

Communicating editor: N. TAKAHATA

We mapped 633 markers (488 AFLPs, 28 RAPDs, 34 IRSs, 75 ESTs, 4 STSs, and 4 phenotypic markers) for the Medaka Oryzias latipes, a teleost fish of the order Beloniformes. Linkage was determined using a reference typing DNA panel from 39 cell lines derived from backcross progeny. This panel provided unlimited DNA for the accumulation of mapping data. The total map length of Medaka was 1354.5 cM and 24 linkage groups were detected, corresponding to the haploid chromosome number of the organism. Thirteen to 49 markers for each linkage group were obtained. Conserved synteny between Medaka and zebrafish was observed for 2 independent linkage groups. Unlike zebrafish, however, the Medaka linkage map showed obvious restriction of recombination on the linkage group containing the male-determining region (Y) locus compared to the autosomal chromosomes.




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