help button home button Genetics Journal Watch
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Henderson, D. S.
Right arrow Articles by Glover, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Henderson, D. S.
Right arrow Articles by Glover, D. M.
Genetics, Vol. 154, 1721-1733, April 2000, Copyright © 2000

Mutual Correction of Faulty PCNA Subunits in Temperature-Sensitive Lethal mus209 Mutants of Drosophila melanogaster

Daryl S. Hendersona, Ulrich K. Wiegandb, David G. Normanc, and David M. Glovera
a CRC Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom,
b Department of Anatomy and Physiology, University of Dundee, Dundee DD1 5EH, United Kingdom
c Department of Chemistry, University of Dundee, Dundee DD1 5EH, United Kingdom

Corresponding author: Daryl S. Henderson, University of Cambridge, Department of Genetics, Downing St., Cambridge CB2 3EH, United Kingdom., dsh25{at}mole.bio.cam.ac.uk (E-mail)

Communicating editor: V. G. FINNERTY

Proliferating cell nuclear antigen (PCNA) functions in DNA replication as a processivity factor for polymerases {delta} and {epsilon}, and in multiple DNA repair processes. We describe two temperature-sensitive lethal alleles (mus209B1 and mus2092735) of the Drosophila PCNA gene that, at temperatures permissive for growth, result in hypersensitivity to DNA-damaging agents, suppression of position-effect variegation, and female sterility in which ovaries are underdeveloped and do not produce eggs. We show by mosaic analysis that the sterility of mus209B1 is partly due to a failure of germ-line cells to proliferate. Strikingly, mus209B1 and mus2092735 interact to restore partial fertility to heteroallelic females, revealing additional roles for PCNA in ovarian development, meiotic recombination, and embryogenesis. We further show that, although mus209B1 and mus2092735 homozygotes are each defective in repair of transposase-induced DNA double-strand breaks in somatic cells, this defect is substantially reversed in the heteroallelic mutant genotype. These novel mutations map to adjacent sites on the three-dimensional structure of PCNA, which was unexpected in the context of this observed interallelic complementation. These mutations, as well as four others we describe, reveal new relationships between the structure and function of PCNA.




This article has been cited by other articles:


Home page
 GeneticsHome page
J. R. LaRocque, D. L. Dougherty, S. K. Hussain, and J. Sekelsky
Reducing DNA Polymerase {alpha} in the Absence of Drosophila ATR Leads to P53-Dependent Apoptosis and Developmental Defects
Genetics, July 1, 2007; 176(3): 1441 - 1451.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. Prosperi
The fellowship of the rings: distinct pools of proliferating cell nuclear antigen trimer at work
FASEB J, May 1, 2006; 20(7): 833 - 837.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. N. Naryzhny, H. Zhao, and H. Lee
Proliferating Cell Nuclear Antigen (PCNA) May Function as a Double Homotrimer Complex in the Mammalian Cell
J. Biol. Chem., April 8, 2005; 280(14): 13888 - 13894.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
J. M. Claycomb, D. M. MacAlpine, J. G. Evans, S. P. Bell, and T. L. Orr-Weaver
Visualization of replication initiation and elongation in Drosophila
J. Cell Biol., October 28, 2002; 159(2): 225 - 236.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Kaur, C. F. Kostrub, and T. Enoch
Structure-Function Analysis of Fission Yeast Hus1-Rad1-Rad9 Checkpoint Complex
Mol. Biol. Cell, December 1, 2001; 12(12): 3744 - 3758.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Genetics Society of America.