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Mutual Correction of Faulty PCNA Subunits in Temperature-Sensitive Lethal mus209 Mutants of Drosophila melanogaster
Daryl S. Hendersona, Ulrich K. Wiegandb, David G. Normanc, and David M. Gloveraa CRC Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom,
b Department of Anatomy and Physiology, University of Dundee, Dundee DD1 5EH, United Kingdom
c Department of Chemistry, University of Dundee, Dundee DD1 5EH, United Kingdom
Corresponding author: Daryl S. Henderson, University of Cambridge, Department of Genetics, Downing St., Cambridge CB2 3EH, United Kingdom., dsh25{at}mole.bio.cam.ac.uk (E-mail)
Communicating editor: V. G. FINNERTY
and 
, and in multiple DNA repair processes. We describe two temperature-sensitive lethal alleles (mus209B1 and mus2092735) of the Drosophila PCNA gene that, at temperatures permissive for growth, result in hypersensitivity to DNA-damaging agents, suppression of position-effect variegation, and female sterility in which ovaries are underdeveloped and do not produce eggs. We show by mosaic analysis that the sterility of mus209B1 is partly due to a failure of germ-line cells to proliferate. Strikingly, mus209B1 and mus2092735 interact to restore partial fertility to heteroallelic females, revealing additional roles for PCNA in ovarian development, meiotic recombination, and embryogenesis. We further show that, although mus209B1 and mus2092735 homozygotes are each defective in repair of transposase-induced DNA double-strand breaks in somatic cells, this defect is substantially reversed in the heteroallelic mutant genotype. These novel mutations map to adjacent sites on the three-dimensional structure of PCNA, which was unexpected in the context of this observed interallelic complementation. These mutations, as well as four others we describe, reveal new relationships between the structure and function of PCNA.
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