Genetics, Vol. 154, 1577-1586, April 2000, Copyright © 2000

The Aspergillus nidulans uvsB Gene Encodes an ATM-Related Kinase Required for Multiple Facets of the DNA Damage Response

Amy F. Hofmanna and Steven D. Harrisa
a Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06030-3205

Corresponding author: Steven D. Harris, Department of Microbiology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030-3205., sharris{at}nso2.uchc.edu (E-mail)

Communicating editor: R. H. DAVIS

In Aspergillus nidulans, uvsB and uvsD belong to the same epistasis group of DNA repair mutants. Recent observations suggest that these genes are likely to control cell cycle checkpoint responses to DNA damage and incomplete replication. Consistent with this notion, we show here that UVSB is a member of the conserved family of ATM-related kinases. Phenotypic characterization of uvsB mutants shows that they possess defects in additional aspects of the DNA damage response besides checkpoint control, including inhibition of septum formation, regulation of gene expression, and induced mutagenesis. The musN227 mutation partially suppresses the poor growth and DNA damage sensitivity of uvsB mutants. Although musN227 partially suppresses several uvsB defects, it does not restore checkpoint function to uvsB mutants. Notably, the failure of uvsB mutants to restrain septum formation in the presence of DNA damage is suppressed by the musN227 mutation. We propose that UVSB functions as the central regulator of the A. nidulans DNA damage response, whereas MUSN promotes recovery by modulating a subset of the response.




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