help button home button Genetics Infection and Immunity
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marsolier, M.-C.
Right arrow Articles by Mann, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marsolier, M.-C.
Right arrow Articles by Mann, C.
Genetics, Vol. 154, 1523-1532, April 2000, Copyright © 2000

Involvement of the PP2C-Like Phosphatase Ptc2p in the DNA Checkpoint Pathways of Saccharomyces cerevisiae

Marie-Claude Marsoliera, Pascal Roussela, Christophe Leroya, and Carl Manna
a Service de Biochimie et de Génétique Moléculaire, CEA/Saclay, 91191 Gif-Sur-Yvette Cedex, France

Corresponding author: Marie-Claude Marsolier, Service de Biochimie et de Génétique Moléculaire (Bat. 142), CEA/Saclay, F-91191 Gif-Sur-Yvette Cedex, France., marsolie{at}jonas.saclay.cea.fr (E-mail)

Communicating editor: M. JOHNSTON

RAD53 encodes a conserved protein kinase that acts as a central transducer in the DNA damage and the DNA replication checkpoint pathways in Saccharomyces cerevisiae. To identify new elements of these pathways acting with or downstream of RAD53, we searched for genes whose overexpression suppressed the toxicity of a dominant-lethal form of RAD53 and identified PTC2, which encodes a protein phosphatase of the PP2C family. PTC2 overexpression induces hypersensitivity to genotoxic agents in wild-type cells and is lethal to rad53, mec1, and dun1 mutants with low ribonucleotide reductase activity. Deleting PTC2 specifically suppresses the hydroxyurea hypersensitivity of mec1 mutants and the lethality of mec1{Delta}. PTC2 is thus implicated in one or several functions related to RAD53, MEC1, and the DNA checkpoint pathways.




This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
N. Lancelot, G. Charier, J. Couprie, I. Duband-Goulet, B. Alpha-Bazin, E. Quemeneur, E. Ma, M.-C. Marsolier-Kergoat, V. Ropars, J.-B. Charbonnier, et al.
The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA
Nucleic Acids Res., September 27, 2007; 35(17): 5898 - 5912.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Guillemain, E. Ma, S. Mauger, S. Miron, R. Thai, R. Guerois, F. Ochsenbein, and M.-C. Marsolier-Kergoat
Mechanisms of Checkpoint Kinase Rad53 Inactivation after a Double-Strand Break in Saccharomyces cerevisiae
Mol. Cell. Biol., May 1, 2007; 27(9): 3378 - 3389.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Clemenson and M.-C. Marsolier-Kergoat
The Spindle Assembly Checkpoint Regulates the Phosphorylation State of a Subset of DNA Checkpoint Proteins in Saccharomyces cerevisiae
Mol. Cell. Biol., December 15, 2006; 26(24): 9149 - 9161.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Gonzalez, A. Ruiz, R. Serrano, J. Arino, and A. Casamayor
Transcriptional Profiling of the Protein Phosphatase 2C Family in Yeast Provides Insights into the Unique Functional Roles of Ptc1
J. Biol. Chem., November 17, 2006; 281(46): 35057 - 35069.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. A. Sharp, G. Rizki, and P. D. Kaufman
Regulation of Histone Deposition Proteins Asf1/Hir1 by Multiple DNA Damage Checkpoint Kinases in Saccharomyces cerevisiae
Genetics, November 1, 2005; 171(3): 885 - 899.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Dubacq, A. Chevalier, and C. Mann
The Protein Kinase Snf1 Is Required for Tolerance to the Ribonucleotide Reductase Inhibitor Hydroxyurea
Mol. Cell. Biol., March 15, 2004; 24(6): 2560 - 2572.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Agarwal, Z. Tang, H. Yu, and O. Cohen-Fix
Two Distinct Pathways for Inhibiting Pds1 Ubiquitination in Response to DNA Damage
J. Biol. Chem., November 7, 2003; 278(45): 45027 - 45033.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
A. L. Hughes and R. Friedman
Parallel Evolution by Gene Duplication in the Genomes of Two Unicellular Fungi
Genome Res., May 1, 2003; 13(5): 794 - 799.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. L. Pike, A. Hammet, and J. Heierhorst
Role of the N-terminal Forkhead-associated Domain in the Cell Cycle Checkpoint Function of the Rad53 Kinase
J. Biol. Chem., April 20, 2001; 276(17): 14019 - 14026.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Genetics Society of America.