help button home button Genetics J Virology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Graves, J. A.
Right arrow Articles by Henry, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Graves, J. A.
Right arrow Articles by Henry, S. A.
Genetics, Vol. 154, 1485-1495, April 2000, Copyright © 2000

Regulation of the Yeast INO1 Gene: The Products of the INO2, INO4 and OPI1 Regulatory Genes Are Not Required for Repression in Response to Inositol

J. Anthony Gravesa and Susan A. Henryb
a Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
b Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213

Corresponding author: Susan A. Henry, Department of Biological Sciences, Carnegie Mellon University, 4440 Fifth Ave., Pittsburgh, PA 15213., sh4b{at}andrew.cmu.edu (E-mail)

Communicating editor: F. WINSTON

The ino2{Delta}, ino4{Delta}, opi1{Delta}, and sin3{Delta} mutations all affect expression of INO1, a structural gene for inositol-1-phosphate synthase. These same mutations affect other genes of phospholipid biosynthesis that, like INO1, contain the repeated element UASINO (consensus 5' CATGTGAAAT 3'). In this study, we evaluated the effects of these four mutations, singly and in all possible combinations, on growth and expression of INO1. All strains carrying an ino2{Delta} or ino4{Delta} mutation, or both, failed to grow in medium lacking inositol. However, when grown in liquid culture in medium containing limiting amounts of inositol, the opi1{Delta} ino4{Delta} strain exhibited a level of INO1 expression comparable to, or higher than, the wild-type strain growing under the same conditions. Furthermore, INO1 expression in the opi1{Delta} ino4{Delta} strain was repressed in cells grown in medium fully supplemented with both inositol and choline. Similar results were obtained using the opi1{Delta} ino2{Delta} ino4{Delta} strain. Regulation of INO1 was also observed in the absence of the SIN3 gene product. Therefore, while Opi1p, Sin3p, and the Ino2p/Ino4p complex all affect the overall level of INO1 expression in an antagonistic manner, they do not appear to be responsible for transmitting the signal that leads to repression of INO1 in response to inositol. Various models for Opi1p function were tested and no evidence for binding of Opi1p to UASINO, or to Ino2p or Ino4p, was obtained.




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
T. B. Reynolds
The Opi1p Transcription Factor Affects Expression of FLO11, Mat Formation, and Invasive Growth in Saccharomyces cerevisiae.
Eukaryot. Cell, August 1, 2006; 5(8): 1266 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. K. Shirra, S. E. Rogers, D. E. Alexander, and K. M. Arndt
The Snf1 Protein Kinase and Sit4 Protein Phosphatase Have Opposing Functions in Regulating TATA-Binding Protein Association With the Saccharomyces cerevisiae INO1 Promoter
Genetics, April 1, 2005; 169(4): 1957 - 1972.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Jesch, X. Zhao, M. T. Wells, and S. A. Henry
Genome-wide Analysis Reveals Inositol, Not Choline, as the Major Effector of Ino2p-Ino4p and Unfolded Protein Response Target Gene Expression in Yeast
J. Biol. Chem., March 11, 2005; 280(10): 9106 - 9118.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Almaguer, W. Cheng, C. Nolder, and J. Patton-Vogt
Glycerophosphoinositol, a Novel Phosphate Source Whose Transport Is Regulated by Multiple Factors in Saccharomyces cerevisiae
J. Biol. Chem., July 23, 2004; 279(30): 31937 - 31942.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ju, G. Shaltiel, A. Shamir, G. Agam, and M. L. Greenberg
Human 1-D-myo-Inositol-3-phosphate Synthase Is Functional in Yeast
J. Biol. Chem., May 21, 2004; 279(21): 21759 - 21765.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Sreenivas and G. M. Carman
Phosphorylation of the Yeast Phospholipid Synthesis Regulatory Protein Opi1p by Protein Kinase A
J. Biol. Chem., May 30, 2003; 278(23): 20673 - 20680.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
S. Kagiwada and R. Zen
Role of the Yeast VAP Homolog, Scs2p, in INO1 Expression and Phospholipid Metabolism
J. Biochem., April 1, 2003; 133(4): 515 - 522.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Oshiro, S. Rangaswamy, X. Chen, G.-S. Han, J. E. Quinn, and G. M. Carman
Regulation of the DPP1-encoded Diacylglycerol Pyrophosphate (DGPP) Phosphatase by Inositol and Growth Phase. INHIBITION OF DGPP PHOSPHATASE ACTIVITY BY CDP-DIACYLGLYCEROL AND ACTIVATION OF PHOSPHATIDYLSERINE SYNTHASE ACTIVITY BY DGPP
J. Biol. Chem., December 22, 2000; 275(52): 40887 - 40896.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Sreenivas, M. J. Villa-Garcia, S. A. Henry, and G. M. Carman
Phosphorylation of the Yeast Phospholipid Synthesis Regulatory Protein Opi1p by Protein Kinase C
J. Biol. Chem., August 3, 2001; 276(32): 29915 - 29923.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Block-Alper, P. Webster, X. Zhou, L. Supekova, W. H. Wong, P. G. Schultz, and D. I. Meyer
IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast
Mol. Biol. Cell, January 1, 2002; 13(1): 40 - 51.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Genetics Society of America.