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Genetics, Vol. 154, 1451-1461, April 2000, Copyright © 2000

A Recombination Repair Gene of Schizosaccharomyces pombe, rhp57, Is a Functional Homolog of the Saccharomyces cerevisiae RAD57 Gene and Is Phylogenetically Related to the Human XRCC3 Gene

Yasuhiro Tsutsuia, Takashi Morishitaa, Hiroshi Iwasakia,b, Hiroyuki Tohc, and Hideo Shinagawaa
a Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan,
b PRESTO, JST, Suita, Osaka 565-0871, Japan,
c Department of Bioinformatics, Biomolecular Engineering Research Institute, Suita, Osaka 565-0874, Japan

Corresponding author: Hideo Shinagawa, Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan., shinagaw{at}biken.osaka-u.ac.jp (E-mail)

Communicating editor: L. S. SYMINGTON

To identify Schizosaccharomyces pombe genes involved in recombination repair, we identified seven mutants that were hypersensitive to both methyl methanesulfonate (MMS) and {gamma}-rays and that contained mutations that caused synthetic lethality when combined with a rad2 mutation. One of the mutants was used to clone the corresponding gene from a genomic library by complementation of the MMS-sensitive phenotype. The gene obtained encodes a protein of 354 amino acids whose sequence is 32% identical to that of the Rad57 protein of Saccharomyces cerevisiae. An rhp57 (RAD57 homolog of S. pombe) deletion strain was more sensitive to MMS, UV, and {gamma}-rays than the wild-type strain and showed a reduction in the frequency of mitotic homologous recombination. The MMS sensitivity was more severe at lower temperature and was suppressed by the presence of a multicopy plasmid bearing the rhp51 gene. An rhp51 rhp57 double mutant was as sensitive to UV and {gamma}-rays as an rhp51 single mutant, indicating that rhp51 function is epistatic to that of rhp57. These characteristics of the rhp57 mutants are very similar to those of S. cerevisiae rad57 mutants. Phylogenetic analysis suggests that Rhp57 and Rad57 are evolutionarily closest to human Xrcc3 of the RecA/Rad51 family of proteins.




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