Sequence and Structural Diversity of the S Locus Genes From Different Lines With the Same Self-Recognition Specificities in Brassica oleracea

Corresponding author: Makoto Kusaba, Institute of Radiation Breeding, National Institute of Agrobiological Resources, P.O. Box 3, Ohmiya-machi, Naka-gun, Ibaraki-ken, 319-2293, Japan., kusaba{at}irb.affrc.go.jp (E-mail)

Communicating editor: M. K. UYENOYAMA

Self-incompatibility (SI) is a mechanism for preventing self-fertilization in flowering plants. In Brassica, it is controlled by a single multi-allelic locus, S, and it is believed that two highly polymorphic genes in the S locus, SLG and SRK, play central roles in self-recognition in stigmas. SRK is a putative receptor protein kinase, whose extracellular domain exhibits high similarity to SLG. We analyzed two pairs of lines showing cross-incompatibility (S² and S^2-b; S¹³ and S^13-b). In S² and S^2-b, SRKs were more highly conserved than SLGs. This was also the case with S¹³ and S^13-b. This suggests that the SRKs of different lines must be conserved for the lines to have the same self-recognition specificity. In particular, SLG^2-b showed only 88.5% identity to SLG², which is comparable to that between the SLGs of different S haplotypes, while SRK^2-b showed 97.3% identity to SRK² in the S domain. These findings suggest that the SLGs in these S haplotypes are not important for self-recognition in SI.

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