A Genetic Test to Determine the Origin of Maternal Transmission Ratio Distortion: Meiotic Drive at the Mouse Om Locus

A Genetic Test to Determine the Origin of Maternal Transmission Ratio Distortion: Meiotic Drive at the Mouse Om Locus

Fernando Pardo-Manuel de Villena^a, Elena de la Casa-Esperón^a, Tammi L. Briscoe^a, and Carmen Sapienza^a,b
^a Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140
^b Department of Pathology and Laboratory Medicine, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

Corresponding author: Carmen Sapienza, Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 N. Broad St., Philadelphia, PA 19140., sapienza{at}unix.temple.edu (E-mail)

Communicating editor: C.-I WU

We have shown previously that the progeny of crosses betweenheterozygous females and C57BL/6 males show transmission ratiodistortion at the Om locus on mouse chromosome 11. This resulthas been replicated in several independent experiments. Herewe show that the distortion maps to a single locus on chromosome11, closely linked to Om, and that gene conversion is not implicatedin the origin of this phenomenon. To further investigate theorigin of the transmission ratio distortion we generated a testusing the well-known effect of recombination on maternal meioticdrive. The genetic test presented here discriminates betweenunequal segregation of alleles during meiosis and lethality,based on the analysis of genotype at both the distorted locusand the centromere of the same chromosome. We used this testto determine the cause of the transmission ratio distortionobserved at the Om locus. Our results indicate that transmissionratio distortion at Om is due to unequal segregation of allelesto the polar body at the second meiotic division. Because thepresence of segregation distortion at Om also depends on thegenotype of the sire, our results confirm that the sperm caninfluence segregation of maternal chromosomes to the secondpolar body.

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