help button home button Genetics J Bacteriology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hu, Z.
Right arrow Articles by Michels, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hu, Z.
Right arrow Articles by Michels, C. A.
Genetics, Vol. 154, 121-132, January 2000, Copyright © 2000

Analysis of the Mechanism by Which Glucose Inhibits Maltose Induction of MAL Gene Expression in Saccharomyces

Zhen Hua, Yingzi Yuea, Hua Jiangb, Bin Zhangb, Peter W. Sherwoodc, and Corinne A. Michelsa,b
a Department of Biochemistry, Queens College and Graduate School of CUNY, Flushing, New York 11367
b Department of Biology, Queens College and Graduate School of CUNY, Flushing, New York 11367
c Department of Genetics and Development, Columbia University, New York, New York 10032

Corresponding author: Corinne A. Michels, Department of Biology, Queens College, Flushing, NY 11367., corinne_michels{at}qc.edu (E-mail)

Communicating editor: M. JOHNSTON

Expression of the MAL genes required for maltose fermentation in Saccharomyces cerevisiae is induced by maltose and repressed by glucose. Maltose-inducible regulation requires maltose permease and the MAL-activator protein, a DNA-binding transcription factor encoded by MAL63 and its homologues at the other MAL loci. Previously, we showed that the Mig1 repressor mediates glucose repression of MAL gene expression. Glucose also blocks MAL-activator-mediated maltose induction through a Mig1p-independent mechanism that we refer to as glucose inhibition. Here we report the characterization of this process. Our results indicate that glucose inhibition is also Mig2p independent. Moreover, we show that neither overexpression of the MAL-activator nor elimination of inducer exclusion is sufficient to relieve glucose inhibition, suggesting that glucose acts to inhibit induction by affecting maltose sensing and/or signaling. The glucose inhibition pathway requires HXK2, REG1, and GSF1 and appears to overlap upstream with the glucose repression pathway. The likely target of glucose inhibition is Snf1 protein kinase. Evidence is presented indicating that, in addition to its role in the inactivation of Mig1p, Snf1p is required post-transcriptionally for the synthesis of maltose permease whose function is essential for maltose induction.




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
J. Houghton-Larsen and A. Brandt
Fermentation of High Concentrations of Maltose by Saccharomyces cerevisiae Is Limited by the COMPASS Methylation Complex
Appl. Envir. Microbiol., November 1, 2006; 72(11): 7176 - 7182.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
G. M. Santangelo
Glucose Signaling in Saccharomyces cerevisiae
Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 253 - 282.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
N. Gadura, L. C. Robinson, and C. A. Michels
Glc7-Reg1 Phosphatase Signals to Yck1,2 Casein Kinase 1 to Regulate Transport Activity and Glucose-Induced Inactivation of Saccharomyces Maltose Permease
Genetics, March 1, 2006; 172(3): 1427 - 1439.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Liu, X. Xu, S. Singh-Rodriguez, Y. Zhao, and M.-H. Kuo
Histone H3 Ser10 Phosphorylation-Independent Function of Snf1 and Reg1 Proteins Rescues a gcn5- Mutant in HIS3 Expression
Mol. Cell. Biol., December 1, 2005; 25(23): 10566 - 10579.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
C. Wiedemuth and K. D. Breunig
Role of Snf1p in Regulation of Intracellular Sorting of the Lactose and Galactose Transporter Lac12p in Kluyveromyces lactis
Eukaryot. Cell, April 1, 2005; 4(4): 716 - 721.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
X. Wang and C. A. Michels
Mutations in SIN4 and RGR1 Cause Constitutive Expression of MAL Structural Genes in Saccharomyces cerevisiae
Genetics, October 1, 2004; 168(2): 747 - 757.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. CEULEMANS and M. BOLLEN
Functional Diversity of Protein Phosphatase-1, a Cellular Economizer and Reset Button
Physiol Rev, January 1, 2004; 84(1): 1 - 39.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Genetics Society of America.