Genetics, Vol. 153, 1929-1948, December 1999, Copyright © 1999

The Mla (Powdery Mildew) Resistance Cluster Is Associated With Three NBS-LRR Gene Families and Suppressed Recombination Within a 240-kb DNA Interval on Chromosome 5S (1HS) of Barley

Fusheng Weia,b, Karin Gobelman-Wernerb,c, Shaun M. Morrollb,c, Joachim Kurthd, Long Maoe, Rod Winge, Dario Leisterd, Paul Schulze-Lefertd, and Roger P. Wisea,b,c
a Interdepartmental Genetics Program, USDA-ARS, Iowa State University, Ames, Iowa 50011-1020,
b Department of Plant Pathology, USDA-ARS, Iowa State University, Ames, Iowa 50011-1020,
c Corn Insects and Crop Genetics Research Unit, USDA-ARS, Iowa State University, Ames, Iowa 50011-1020,
d Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom
e Clemson University Genomics Institute, Clemson, South Carolina 29634

Corresponding author: Roger P. Wise, USDA-ARS-Corn Insects and Crop Genetics Research Unit, Department of Plant Pathology, 409 Bessey Hall, Iowa State University, Ames, IA 50011-1020., rpwise{at}iastate.edu (E-mail)

Communicating editor: B. S. GILL

Powdery mildew of barley, caused by Erysiphe graminis f. sp. hordei, is a model system for investigating the mechanism of gene-for-gene interaction between large-genome cereals and obligate-fungal pathogens. A large number of loci that confer resistance to this disease are located on the short arm of chromosome 5(1H). The Mla resistance-gene cluster is positioned near the telomeric end of this chromosome arm. AFLP-, RAPD-, and RFLP-derived markers were used to saturate the Mla region in a high-resolution recombinant population segregating for the (Mla6 + Mla14) and (Mla13 + Ml-Ru3) resistance specificities. These tightly linked genetic markers were used to identify and develop a physical contig of YAC and BAC clones spanning the Mla cluster. Three distinct NBS-LRR resistance-gene homologue (RGH) families were revealed via computational analysis of low-pass and BAC-end sequence data derived from Mla-spanning clones. Genetic and physical mapping delimited the Mla-associated, NBS-LRR gene families to a 240-kb interval. Recombination within the RGH families was at least 10-fold less frequent than between markers directly adjacent to the Mla cluster.




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