DNA Polymorphism, Haplotype Structure and Balancing Selection in the Leavenworthia PgiC Locus

Corresponding author: Dmitry A. Filatov, Institute of Cell, Animal, and Population Biology, King's Bldgs., West Mains Rd., University of Edinburgh, Edinburgh EH9 3JT, United Kingdom., dmitry.filatov{at}ed.ac.uk (E-mail)

Communicating editor: W. STEPHAN

A study of DNA polymorphism and divergence was conducted for the cytosolic phosphoglucose isomerase (PGI:E.C.5.3.1.9) gene of five species of the mustard genus Leavenworthia: Leavenworthia stylosa, L. alabamica, L. crassa, L. uniflora, and L. torulosa. Sequences of an internal 2.3-kb PgiC gene region spanning exons 6–16 were obtained from 14 L. stylosa plants from two natural populations and from one to several plants for each of the other species. The level of nucleotide polymorphism in L. stylosa PgiC gene was quite high ( = 0.051, = 0.052). Although recombination is estimated to be high in this locus, extensive haplotype structure was observed for the entire 2.3-kb region. The L. stylosa sequences fall into at least two groups, distinguished by the presence of several indels and nucleotide substitutions, and one of the three charge change nucleotide replacements within the region sequenced correlates with the haplotypes. The differences between the haplotypes are older than between the species, and the haplotypes are still segregating in at least two of five species studied. There is no evidence of recent or ancient population subdivision that could maintain distinct haplotypes. The age of the haplotypes and the results of Kelly's Z_nS and Wall's B and Q tests with recombination suggest that the haplotypes are maintained due to balancing selection at or near this locus.

This article has been cited by other articles:

				L. E. Wallace, S. G. Weller, W. L. Wagner, A. K. Sakai, and M. Nepokroeff Phylogeographic patterns and demographic history of Schiedea globosa (Caryophyllaceae) on the Hawaiian Islands Am. J. Botany, May 1, 2009; 96(5): 958 - 967. [Abstract] [Full Text] [PDF]

				N. E. Rank, D. A. Bruce, D. M. McMillan, C. Barclay, and E. P. Dahlhoff Phosphoglucose isomerase genotype affects running speed and heat shock protein expression after exposure to extreme temperatures in a montane willow beetle J. Exp. Biol., March 1, 2007; 210(5): 750 - 764. [Abstract] [Full Text] [PDF]

				S. C. Gonzalez-Martinez, E. Ersoz, G. R. Brown, N. C. Wheeler, and D. B. Neale DNA Sequence Variation and Selection of Tag Single-Nucleotide Polymorphisms at Candidate Genes for Drought-Stress Response in Pinus taeda L. Genetics, March 1, 2006; 172(3): 1915 - 1926. [Abstract] [Full Text] [PDF]

				C. W. Wheat, W. B. Watt, D. D. Pollock, and P. M. Schulte From DNA to Fitness Differences: Sequences and Structures of Adaptive Variants of Colias Phosphoglucose Isomerase (PGI) Mol. Biol. Evol., March 1, 2006; 23(3): 499 - 512. [Abstract] [Full Text] [PDF]

				E. S. Balakirev, V. R. Chechetkin, V. V. Lobzin, and F. J. Ayala Entropy and GC Content in the {beta}-esterase Gene Cluster of the Drosophila melanogaster Subgroup Mol. Biol. Evol., October 1, 2005; 22(10): 2063 - 2072. [Abstract] [Full Text] [PDF]

				J. W. Busch The evolution of self-compatibility in geographically peripheral populations of Leavenworthia alabamica (Brassicaceae) Am. J. Botany, September 1, 2005; 92(9): 1503 - 1512. [Abstract] [Full Text] [PDF]

				S. I. Wright and B. S. Gaut Molecular Population Genetics and the Search for Adaptive Evolution in Plants Mol. Biol. Evol., March 1, 2005; 22(3): 506 - 519. [Abstract] [Full Text] [PDF]

				K. S. Caldwell, J. Dvorak, E. S. Lagudah, E. Akhunov, M.-C. Luo, P. Wolters, and W. Powell Sequence Polymorphism in Polyploid Wheat and Their D-Genome Diploid Ancestor Genetics, June 1, 2004; 167(2): 941 - 947. [Abstract] [Full Text] [PDF]

				C. Delye, C. Straub, S. Michel, and V. Le Corre Nucleotide Variability at the Acetyl Coenzyme A Carboxylase Gene and the Signature of Herbicide Selection in the Grass Weed Alopecurus myosuroides (Huds.) Mol. Biol. Evol., May 1, 2004; 21(5): 884 - 892. [Abstract] [Full Text] [PDF]

				E. S. Balakirev and F. J. Ayala Nucleotide Variation in the tinman and bagpipe Homeobox Genes of Drosophila melanogaster Genetics, April 1, 2004; 166(4): 1845 - 1856. [Abstract] [Full Text] [PDF]

				R. Piccinali, M. Aguade, and E. Hasson Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae Mol. Biol. Evol., January 1, 2004; 21(1): 141 - 152. [Abstract] [Full Text] [PDF]

				E. S. Balakirev and F. J. Ayala Nucleotide Variation of the Est-6 Gene Region in Natural Populations of Drosophila melanogaster Genetics, December 1, 2003; 165(4): 1901 - 1914. [Abstract] [Full Text] [PDF]

				D. Charlesworth, B. K. Mable, M. H. Schierup, C. Bartolome, and P. Awadalla Diversity and Linkage of Genes in the Self-Incompatibility Gene Family in Arabidopsis lyrata Genetics, August 1, 2003; 164(4): 1519 - 1535. [Abstract] [Full Text] [PDF]

				T. Kado, H. Yoshimaru, Y. Tsumura, and H. Tachida DNA Variation in a Conifer, Cryptomeria japonica (Cupressaceae sensu lato) Genetics, August 1, 2003; 164(4): 1547 - 1559. [Abstract] [Full Text] [PDF]

				E. S. Balakirev, V. R. Chechetkin, V. V. Lobzin, and F. J. Ayala DNA Polymorphism in the {beta}-Esterase Gene Cluster of Drosophila melanogaster Genetics, June 1, 2003; 164(2): 533 - 544. [Abstract] [Full Text] [PDF]

				G. A. Gomez and E. Hasson Transpecific Polymorphisms in an Inversion Linked Esterase Locus in Drosophila buzzatii Mol. Biol. Evol., March 1, 2003; 20(3): 410 - 423. [Abstract] [Full Text] [PDF]

				U. Lagercrantz, M. Kruskopf Osterberg, and M. Lascoux Sequence Variation and Haplotype Structure at the Putative Flowering-Time Locus COL1 of Brassica nigra Mol. Biol. Evol., September 1, 2002; 19(9): 1474 - 1482. [Abstract] [Full Text] [PDF]

				L. G. S. R. Bauzer, J. S. M. Gesto, N. A. Souza, R. D. Ward, J. G. C. Hamilton, C. P. Kyriacou, and A. A. Peixoto Molecular Divergence in the period Gene Between Two Putative Sympatric Species of the Lutzomyia longipalpis Complex Mol. Biol. Evol., September 1, 2002; 19(9): 1624 - 1627. [Full Text] [PDF]

				D. A. Filatov and D. Charlesworth Substitution Rates in the X- and Y-Linked Genes of the Plants, Silene latifolia and S. dioica Mol. Biol. Evol., June 1, 2002; 19(6): 898 - 907. [Abstract] [Full Text] [PDF]

				A. Graustein, J. M. Gaspar, J. R. Walters, and M. F. Palopoli Levels of DNA Polymorphism Vary With Mating System in the Nematode Genus Caenorhabditis Genetics, May 1, 2002; 161(1): 99 - 107. [Abstract] [Full Text] [PDF]

				J. Vieira Two Divergent Species of the Virilis Group, Drosophila littoralis and Drosophila virilis, Share a Replacement Polymorphism at the fused Locus Mol. Biol. Evol., April 1, 2002; 19(4): 579 - 581. [Full Text] [PDF]

				V. Dvornyk, A. Sirvio, M. Mikkonen, and O. Savolainen Low Nucleotide Diversity at the pal1 Locus in the Widely Distributed Pinus sylvestris Mol. Biol. Evol., February 1, 2002; 19(2): 179 - 188. [Abstract] [Full Text] [PDF]

				D. A. Filatov, V. Laporte, C. Vitte, and D. Charlesworth DNA Diversity in Sex-Linked and Autosomal Genes of the Plant Species Silene latifolia and Silene dioica Mol. Biol. Evol., August 1, 2001; 18(8): 1442 - 1454. [Abstract] [Full Text] [PDF]

				V. Laporte and D. Charlesworth Non-Sex-Linked, Nuclear Cleaved Amplified Polymorphic Sequences in Silene latifolia J. Hered., July 1, 2001; 92(4): 357 - 359. [Abstract] [Full Text] [PDF]

				A. Kawabe, K. Yamane, and N. T. Miyashita DNA Polymorphism at the Cytosolic Phosphoglucose Isomerase (PgiC) Locus of the Wild Plant Arabidopsis thaliana Genetics, November 1, 2000; 156(3): 1339 - 1347. [Abstract] [Full Text]

				J. Vieira and B. Charlesworth Evidence for Selection at the fused Locus of Drosophila virilis Genetics, August 1, 2000; 155(4): 1701 - 1709. [Abstract] [Full Text]

				K. E. Holsinger Reproductive systems and evolution in vascular plants PNAS, June 20, 2000; 97(13): 7037 - 7042. [Abstract] [Full Text] [PDF]