Transposition of the Retrotransposon MAGGY in Heterologous Species of Filamentous Fungi

Corresponding author: Hitoshi Nakayashiki, Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, Rokkodai-cho 1-1, Kobe 657-8501, Japan., hn33{at}cornell.edu (E-mail)

Communicating editor: M. E. ZOLAN

MAGGY is a gypsy-like LTR retrotransposon isolated from the blast fungus Pyricularia grisea (teleomorph, Magnaporthe grisea). We examined transposition of MAGGY in three P. grisea isolates (wheat, finger millet, and crabgrass pathogen), which did not originally possess a MAGGY element, and in two heterologous species of filamentous fungi, Colletotrichum lagenarium and P. zingiberi. Genomic Southern analysis of MAGGY transformants suggested that transposition of MAGGY occurred in all filamentous fungi tested. In contrast, no transposition was observed in any transformants with a modified MAGGY containing a 513-bp deletion in the reverse transcriptase domain. When a MAGGY derivative carrying an artificial intron was introduced into the wheat isolate of P. grisea and C. lagenarium, loss of the intron was observed. These results showed that MAGGY can undergo autonomous RNA-mediated transposition in heterologous filamentous fungi. The frequency of transposition differed among fungal species. MAGGY transposed actively in the wheat isolate of P. grisea and P. zingiberi, but transposition in C. lagenarium appeared to be rare. This is the first report that demonstrates active transposition of a fungal transposable element in heterologous hosts. Possible usage of MAGGY as a genetic tagging tool in filamentous fungi is discussed.

This article has been cited by other articles:

				X. Gao, Y. Hou, H. Ebina, H. L. Levin, and D. F. Voytas Chromodomains direct integration of retrotransposons to heterochromatin Genome Res., March 1, 2008; 18(3): 359 - 369. [Abstract] [Full Text] [PDF]

				T. Murata, N. Kadotani, M. Yamaguchi, Y. Tosa, S. Mayama, and H. Nakayashiki siRNA-dependent and -independent post-transcriptional cosuppression of the LTR-retrotransposon MAGGY in the phytopathogenic fungus Magnaporthe oryzae Nucleic Acids Res., September 25, 2007; 35(18): 5987 - 5994. [Abstract] [Full Text] [PDF]

				N. Kadotani, H. Nakayashiki, Y. Tosa, and S. Mayama One of the Two Dicer-like Proteins in the Filamentous Fungi Magnaporthe oryzae Genome Is Responsible for Hairpin RNA-triggered RNA Silencing and Related Small Interfering RNA Accumulation J. Biol. Chem., October 22, 2004; 279(43): 44467 - 44474. [Abstract] [Full Text] [PDF]

				H. Nakayashiki, H. Matsuo, I. Chuma, K. Ikeda, S. Betsuyaku, M. Kusaba, Y. Tosa, and S. Mayama Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains Nucleic Acids Res., October 15, 2001; 29(20): 4106 - 4113. [Abstract] [Full Text] [PDF]

				H. Nakayashiki, K. Ikeda, Y. Hashimoto, Y. Tosa, and S. Mayama Methylation is not the main force repressing the retrotransposon MAGGY in Magnaporthe grisea Nucleic Acids Res., March 15, 2001; 29(6): 1278 - 1284. [Abstract] [Full Text] [PDF]