Genetics, Vol. 153, 653-664, October 1999, Copyright © 1999

Appearance and Properties of L-Sorbose-Utilizing Mutants of Candida albicans Obtained on a Selective Plate

Guilhem Janbona, Fred Shermana, and Elena Rustchenkoa
a Department of Biochemistry and Biophysics, University of Rochester Medical School, Rochester, New York 14642

Corresponding author: Elena Rustchenko, Department of Biochemistry and Biophysics, Box 712, University of Rochester Medical School, Rochester, NY 14642., elena_bulgac{at}urmc.rochester.edu (E-mail)

Communicating editor: P. L. FOSTER

This is the first report that adaptive mutagenesis can arise by chromosomal nondisjunction, a phenomenon previously associated exclusively with DNA alterations. We previously uncovered a novel regulatory mechanism in Candida albicans in which the assimilation of an alternative sugar, L-sorbose, was determined by copy number of chromosome 5, such that monosomic strains utilized L-sorbose, whereas disomic strains did not. We present evidence that this formation of monosomy of chromosome 5, which is apparently a result of nondisjunction, appeared with increased frequencies after a selective condition was applied, i.e., by adaptive mutagenesis. The rate of formation of L-sorbose-utilizing mutants per viable cell per day ranged from 10-6 at the initial time of detection to 10-2 after 4 days of incubation on the selective plate.




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
L. Edwards-Ingram, P. Gitsham, N. Burton, G. Warhurst, I. Clarke, D. Hoyle, S. G. Oliver, and L. Stateva
Genotypic and Physiological Characterization of Saccharomyces boulardii, the Probiotic Strain of Saccharomyces cerevisiae
Appl. Envir. Microbiol., April 15, 2007; 73(8): 2458 - 2467.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
P. R. Lephart and P. T. Magee
Effect of the Major Repeat Sequence on Mitotic Recombination in Candida albicans
Genetics, December 1, 2006; 174(4): 1737 - 1744.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
N. Chauhan, T. Ciudad, A. Rodriguez-Alejandre, G. Larriba, R. Calderone, and E. Andaluz
Virulence and Karyotype Analyses of rad52 Mutants of Candida albicans: Regeneration of a Truncated Chromosome of a Reintegrant Strain (rad52/RAD52) in the Host
Infect. Immun., December 1, 2005; 73(12): 8069 - 8078.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. S. Ibrahim, B. B. Magee, D. C. Sheppard, M. Yang, S. Kauffman, J. Becker, J. E. Edwards Jr., and P. T. Magee
Effects of Ploidy and Mating Type on Virulence of Candida albicans
Infect. Immun., November 1, 2005; 73(11): 7366 - 7374.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. A. Kabir, A. Ahmad, J. R. Greenberg, Y.-K. Wang, and E. Rustchenko
Loss and gain of chromosome 5 controls growth of Candida albicans on sorbose due to dispersed redundant negative regulators
PNAS, August 23, 2005; 102(34): 12147 - 12152.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
P. Sampaio, L. Gusmao, A. Correia, C. Alves, A. G. Rodrigues, C. Pina-Vaz, A. Amorim, and C. Pais
New Microsatellite Multiplex PCR for Candida albicans Strain Typing Reveals Microevolutionary Changes
J. Clin. Microbiol., August 1, 2005; 43(8): 3869 - 3876.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
P. R. Lephart, H. Chibana, and P. T. Magee
Effect of the Major Repeat Sequence on Chromosome Loss in Candida albicans
Eukaryot. Cell, April 1, 2005; 4(4): 733 - 741.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Sanyal, M. Baum, and J. Carbon
Centromeric DNA sequences in the pathogenic yeast Candida albicans are all different and unique
PNAS, August 3, 2004; 101(31): 11374 - 11379.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
D. Wunder, J. Dong, D. Baev, and M. Edgerton
Human Salivary Histatin 5 Fungicidal Action Does Not Induce Programmed Cell Death Pathways in Candida albicans
Antimicrob. Agents Chemother., January 1, 2004; 48(1): 110 - 115.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
N. Zhang, A. L. Harrex, B. R. Holland, L. E. Fenton, R. D. Cannon, and J. Schmid
Sixty Alleles of the ALS7 Open Reading Frame in Candida albicans: ALS7 Is a Hypermutable Contingency Locus
Genome Res., September 1, 2003; 13(9): 2005 - 2017.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
T. R. Rustad, D. A. Stevens, M. A. Pfaller, and T. C. White
Homozygosity at the Candida albicans MTL locus associated with azole resistance
Microbiology, April 1, 2002; 148(4): 1061 - 1072.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. Enloe, A. Diamond, and A. P. Mitchell
A Single-Transformation Gene Function Test in Diploid Candida albicans
J. Bacteriol., October 15, 2000; 182(20): 5730 - 5736.
[Abstract] [Full Text]

Home page
 MicrobiologyHome page
J. F. Ernst
Transcription factors in Candida albicans - environmental control of morphogenesis
Microbiology, August 1, 2000; 146(8): 1763 - 1774.
[Full Text]