Genetics, Vol. 153, 445-452, September 1999, Copyright © 1999

Analysis and Mapping of Gene Families Encoding ß-1,3-Glucanases of Soybean

Wei Jina, Harry T. Hornera, Reid G. Palmerb, and Randy C. Shoemakerb
a Interdepartmental Plant Physiology Program and Department of Botany, Zoology/Genetics and USDA ARS CICG Research Unit, Iowa State University, Ames, Iowa 50011
b Departments of Agronomy, Zoology/Genetics and USDA ARS CICG Research Unit, Iowa State University, Ames, Iowa 50011

Corresponding author: Harry T. Horner, Department of Botany, Iowa State University, Ames, IA 50011-1020., hth{at}iastate.edu (E-mail)

Communicating editor: V. SUNDARESAN

Oligonucleotide primers designed for conserved sequences from coding regions of ß-1,3-glucanase genes from different species were used to amplify related sequences from soybean [Glycine max (L.) Merr.]. Sequencing and cross-hybridization of amplification products indicated that at least 12 classes of ß-1,3-glucanase genes exist in the soybean. Members of classes mapped to 34 loci on five different linkage groups using an F2 population of 56 individuals. ß-1,3-Glucanase genes are clustered onto regions of five linkage groups. Data suggest that more closely related genes are clustered together on one linkage group or on duplicated regions of linkage groups. Northern blot analyses performed on total RNA from root, stem, leaf, pod, flower bud, and hypocotyl using DNA probes for the different classes of ß-1,3-glucanase genes revealed that the mRNA levels of all classes were low in young leaves. SGlu2, SGlu4, SGlu7, and SGlu12 mRNA were highly accumulated in young roots and hypocotyls. SGlu7 mRNA also accumulated in pods and flower buds.




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