Conservation of ARS Elements and Chromosomal DNA Replication Origins on Chromosomes III of Saccharomyces cerevisiae and S. carlsbergensis

Conservation of ARS Elements and Chromosomal DNA Replication Origins on Chromosomes III of Saccharomyces cerevisiae and S. carlsbergensis

Chen Yang^a, James F. Theis^a, and Carol S. Newlon^a
^a Department of Microbiology and Molecular Genetics, New Jersey Medical School and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103

Corresponding author: Carol S. Newlon, Department of Microbiology and Molecular Genetics, UMDNJ–New Jersey Medical School, 185 S. Orange Ave., Newark, NJ 07103., newlon{at}umdnj.edu (E-mail)

Communicating editor: M. JOHNSTON

DNA replication origins, specified by ARS elements in Saccharomycescerevisiae, play an essential role in the stable transmissionof chromosomes. Little is known about the evolution of ARS elements.We have isolated and characterized ARS elements from a chromosomeIII recovered from an alloploid Carlsberg brewing yeast thathas diverged from its S. cerevisiae homeologue. The positionsof seven ARS elements identified in this S. carlsbergensis chromosomeare conserved: they are located in intergenic regions flankedby open reading frames homologous to those that flank sevenARS elements of the S. cerevisiae chromosome. The S. carlsbergensisARS elements were active both in S. cerevisiae and S. monacensis,which has been proposed to be the source of the diverged genomepresent in brewing yeast. Moreover, their function as chromosomalreplication origins correlated strongly with the activity ofS. cerevisiae ARS elements, demonstrating the conservation ofARS activity and replication origin function in these two species.

This article has been cited by other articles:

A. Dershowitz, M. Snyder, M. Sbia, J. H. Skurnick, L. Y. Ong, and C. S. Newlon
Linear Derivatives of Saccharomyces cerevisiae Chromosome III Can Be Maintained in the Absence of Autonomously Replicating Sequence Elements
Mol. Cell. Biol., July 1, 2007; 27(13): 4652 - 4663.
[Abstract] [Full Text] [PDF]

A. B. Barton and D. B. Kaback
Telomeric Silencing of an Open Reading Frame in Saccharomyces cerevisiae
Genetics, June 1, 2006; 173(2): 1169 - 1173.
[Abstract] [Full Text] [PDF]

A. B. Barton, Y. Su, J. Lamb, D. Barber, and D. B. Kaback
A Function for Subtelomeric DNA in Saccharomyces cerevisiae
Genetics, October 1, 2003; 165(2): 929 - 934.
[Abstract] [Full Text] [PDF]

J. F. Theis and C. S. Newlon
Two Compound Replication Origins in Saccharomyces cerevisiae Contain Redundant Origin Recognition Complex Binding Sites
Mol. Cell. Biol., April 15, 2001; 21(8): 2790 - 2801.
[Abstract] [Full Text]

J. F. Theis, C. Yang, C. B. Schaefer, and C. S. Newlon
DNA Sequence and Functional Analysis of Homologous ARS Elements of Saccharomyces cerevisiae and S. carlsbergensis
Genetics, July 1, 1999; 152(3): 943 - 952.
[Abstract] [Full Text]

				A. B. Barton and D. B. Kaback Telomeric Silencing of an Open Reading Frame in Saccharomyces cerevisiae Genetics, June 1, 2006; 173(2): 1169 - 1173. [Abstract] [Full Text] [PDF]

				A. B. Barton, Y. Su, J. Lamb, D. Barber, and D. B. Kaback A Function for Subtelomeric DNA in Saccharomyces cerevisiae Genetics, October 1, 2003; 165(2): 929 - 934. [Abstract] [Full Text] [PDF]

				J. F. Theis and C. S. Newlon Two Compound Replication Origins in Saccharomyces cerevisiae Contain Redundant Origin Recognition Complex Binding Sites Mol. Cell. Biol., April 15, 2001; 21(8): 2790 - 2801. [Abstract] [Full Text]

				J. F. Theis, C. Yang, C. B. Schaefer, and C. S. Newlon DNA Sequence and Functional Analysis of Homologous ARS Elements of Saccharomyces cerevisiae and S. carlsbergensis Genetics, July 1, 1999; 152(3): 943 - 952. [Abstract] [Full Text]