A Molecular Description of Mutations Affecting the Pollen Component of the Nicotiana alata S locus

Corresponding author: A. E. Clarke, Plant Cell Biology Research Center, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia., a.clarke{at}botany.unimelb.edu.au (E-mail)

Communicating editor: J. CHORY

Mutations affecting the self-incompatibility response of Nicotiana alata were generated by irradiation. Mutants in the M₁ generation were selected on the basis of pollen tube growth through an otherwise incompatible pistil. Twelve of the 18 M₁ plants obtained from the mutagenesis screen were self-compatible. Eleven self-compatible plants had mutations affecting only the pollen function of the S locus (pollen-part mutants). The remaining self-compatible plant had a mutation affecting only the style function of the S locus (style-part mutant). Cytological examination of the pollen-part mutant plants revealed that 8 had an extra chromosome (2n + 1) and 3 did not. The pollen-part mutation in 7 M₁ plants was followed in a series of crosses. DNA blot analysis using probes for S-RNase genes (encoding the style function of the S locus) indicated that the pollen-part mutation was associated with an extra S allele in 4 M₁ plants. In 3 of these plants, the extra S allele was located on the additional chromosome. There was no evidence of an extra S allele in the 3 remaining M₁ plants. The breakdown of self-incompatibility in plants with an extra S allele is discussed with reference to current models of the molecular basis of self-incompatibility.

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