Molecular Characterization of Mutant Alleles of the DNA Repair/Basal Transcription Factor haywire/ERCC3 in Drosophila

Molecular Characterization of Mutant Alleles of the DNA Repair/Basal Transcription Factor haywire/ERCC3 in Drosophila

Leslie C. Mounkes^a,b and Margaret T. Fuller^b
^a Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309
^b Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, California 94309

Corresponding author: Margaret T. Fuller, Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305-5427., fuller{at}cmgm.stanford.edu (E-mail)

Communicating editor: R. S. HAWLEY

The haywire gene of Drosophila encodes a putative helicase essentialfor transcription and nucleotide excision repair. A haywireallele encoding a dominant acting poison product, lethal alleles,and viable but UV-sensitive alleles isolated as revertants ofthe dominant acting poison allele were molecularly characterized.Sequence analysis of lethal haywire alleles revealed the importanceof the nucleotide-binding domain, suggesting an essential rolefor ATPase activity. The viable hay^nc2 allele, which encodesa poison product, has a single amino acid change in conservedhelicase domain VI. This mutation results in accumulation ofa 68-kD polypeptide that is much more abundant than the wild-typehaywire protein.

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