help button home button Genetics J Biol Chem
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, W.
Right arrow Articles by Jinks-Robertson, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, W.
Right arrow Articles by Jinks-Robertson, S.
Genetics, Vol. 151, 1299-1313, April 1999, Copyright © 1999

The Role of the Mismatch Repair Machinery in Regulating Mitotic and Meiotic Recombination Between Diverged Sequences in Yeast

Wenliang Chena and Sue Jinks-Robertsona,b
a Graduate Program in Genetics and Molecular Biology, Emory University, Atlanta, Georgia 30322
b Department of Biology, Emory University, Atlanta, Georgia 30322

Corresponding author: Sue Jinks-Robertson, Department of Biology, 1510 Clifton Rd., Emory University, Atlanta, GA 30322., jinks{at}biology.emory.edu (E-mail)

Communicating editor: M. LICHTEN

Nonidentical recombination substrates recombine less efficiently than do identical substrates in yeast, and much of this inhibition can be attributed to action of the mismatch repair (MMR) machinery. In this study an intron-based inverted repeat assay system has been used to directly compare the rates of mitotic and meiotic recombination between pairs of 350-bp substrates varying from 82% to 100% in sequence identity. The recombination rate data indicate that sequence divergence impacts mitotic and meiotic recombination similarly, although subtle differences are evident. In addition to assessing recombination rates as a function of sequence divergence, the endpoints of mitotic and meiotic recombination events involving 94%-identical substrates were determined by DNA sequencing. The endpoint analysis indicates that the extent of meiotic heteroduplex DNA formed in a MMR-defective strain is 65% longer than that formed in a wild-type strain. These data are consistent with a model in which the MMR machinery interferes with the formation and/or extension of heteroduplex intermediates during recombination.




This article has been cited by other articles:


Home page
 GeneticsHome page
J. E. Stone, R. G. Ozbirn, T. D. Petes, and S. Jinks-Robertson
Role of Proliferating Cell Nuclear Antigen Interactions in the Mismatch Repair-Dependent Processing of Mitotic and Meiotic Recombination Intermediates in Yeast
Genetics, March 1, 2008; 178(3): 1221 - 1236.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. A. Smith, L. A. Bannister, V. Bhattacharjee, Y. Wang, B. C. Waldman, and A. S. Waldman
Accurate Homologous Recombination Is a Prominent Double-Strand Break Repair Pathway in Mammalian Chromosomes and Is Modulated by Mismatch Repair Protein Msh2
Mol. Cell. Biol., November 15, 2007; 27(22): 7816 - 7827.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. Sun and J. Xu
Genetic Analyses of a Hybrid Cross Between Serotypes A and D Strains of the Human Pathogenic Fungus Cryptococcus neoformans
Genetics, November 1, 2007; 177(3): 1475 - 1486.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. L. Barnes and R. McCulloch
Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases
Nucleic Acids Res., May 11, 2007; 35(10): 3478 - 3493.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Yang, E. B. Goldsmith, Y. Lin, B. C. Waldman, V. Kaza, and A. S. Waldman
Genetic Exchange Between Homeologous Sequences in Mammalian Chromosomes Is Averted by Local Homology Requirements for Initiation and Resolution of Recombination
Genetics, September 1, 2006; 174(1): 135 - 144.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. D. Yandeau-Nelson, Y. Xia, J. Li, M. G. Neuffer, and P. S. Schnable
Unequal Sister Chromatid and Homolog Recombination at a Tandem Duplication of the a1 Locus in Maize
Genetics, August 1, 2006; 173(4): 2211 - 2226.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Nicholson, R. M. Fabbri, J. W. Reeves, and G. F. Crouse
The Effects of Mismatch Repair and RAD1 Genes on Interchromosomal Crossover Recombination in Saccharomyces cerevisiae
Genetics, June 1, 2006; 173(2): 647 - 659.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Daikoku, A. Kudoh, Y. Sugaya, S. Iwahori, N. Shirata, H. Isomura, and T. Tsurumi
Postreplicative Mismatch Repair Factors Are Recruited to Epstein-Barr Virus Replication Compartments
J. Biol. Chem., April 21, 2006; 281(16): 11422 - 11430.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. I. Khrustaleva, P. E. de Melo, A. W. van Heusden, and C. Kik
The Integration of Recombination and Physical Maps in a Large-Genome Monocot Using Haploid Genome Analysis in a Trihybrid Allium Population
Genetics, March 1, 2005; 169(3): 1673 - 1685.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
T. Goldfarb and E. Alani
Distinct Roles for the Saccharomyces cerevisiae Mismatch Repair Proteins in Heteroduplex Rejection, Mismatch Repair and Nonhomologous Tail Removal
Genetics, February 1, 2005; 169(2): 563 - 574.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. D. Yandeau-Nelson, Q. Zhou, H. Yao, X. Xu, B. J. Nikolau, and P. S. Schnable
MuDR Transposase Increases the Frequency of Meiotic Crossovers in the Vicinity of a Mu Insertion in the Maize a1 Gene
Genetics, February 1, 2005; 169(2): 917 - 929.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. M. Spell and S. Jinks-Robertson
Examination of the Roles of Sgs1 and Srs2 Helicases in the Enforcement of Recombination Fidelity in Saccharomyces cerevisiae
Genetics, December 1, 2004; 168(4): 1855 - 1865.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. Opperman, E. Emmanuel, and A. A. Levy
The Effect of Sequence Divergence on Recombination Between Direct Repeats in Arabidopsis
Genetics, December 1, 2004; 168(4): 2207 - 2215.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Sugawara, T. Goldfarb, B. Studamire, E. Alani, and J. E. Haber
Heteroduplex rejection during single-strand annealing requires Sgs1 helicase and mismatch repair proteins Msh2 and Msh6 but not Pms1
PNAS, June 22, 2004; 101(25): 9315 - 9320.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. G. Jelesko, K. Carter, W. Thompson, Y. Kinoshita, and W. Gruissem
Meiotic Recombination Between Paralogous RBCSB Genes on Sister Chromatids of Arabidopsis thaliana
Genetics, February 1, 2004; 166(2): 947 - 957.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. M. Spell and S. Jinks-Robertson
Role of Mismatch Repair in the Fidelity of RAD51- and RAD59-Dependent Recombination in Saccharomyces cerevisiae
Genetics, December 1, 2003; 165(4): 1733 - 1744.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. V. Abdelnoor, R. Yule, A. Elo, A. C. Christensen, G. Meyer-Gauen, and S. A. Mackenzie
Substoichiometric shifting in the plant mitochondrial genome is influenced by a gene homologous to MutS
PNAS, May 13, 2003; 100(10): 5968 - 5973.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. M. Stark and M. Jasin
Extensive Loss of Heterozygosity Is Suppressed during Homologous Repair of Chromosomal Breaks
Mol. Cell. Biol., January 15, 2003; 23(2): 733 - 743.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. Welz-Voegele, J. E. Stone, P. T. Tran, H. M. Kearney, R. M. Liskay, T. D. Petes, and S. Jinks-Robertson
Alleles of the Yeast PMS1 Mismatch-Repair Gene That Differentially Affect Recombination- and Replication-Related Processes
Genetics, November 1, 2002; 162(3): 1131 - 1145.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. J. Raynard and M. D. Baker
Incorporation of Large Heterologies Into Heteroduplex DNA During Double-Strand-Break Repair in Mouse Cells
Genetics, October 1, 2002; 162(2): 977 - 985.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
J. A. Birdsell
Integrating Genomics, Bioinformatics, and Classical Genetics to Study the Effects of Recombination on Genome Evolution
Mol. Biol. Evol., July 1, 2002; 19(7): 1181 - 1197.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
G. P. Copenhaver, E. A. Housworth, and F. W. Stahl
Crossover Interference in Arabidopsis
Genetics, April 1, 2002; 160(4): 1631 - 1639.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Allen, A. Kurimasa, M. A. Brenneman, D. J. Chen, and J. A. Nickoloff
DNA-dependent protein kinase suppresses double-strand break-induced and spontaneous homologous recombination
PNAS, March 19, 2002; 99(6): 3758 - 3763.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C.-G. Gendrel and M. Dutreix
(CA/TG) Microsatellite Sequences Escape the Inhibition of Recombination by Mismatch Repair in Saccharomyces cerevisiae
Genetics, December 1, 2001; 159(4): 1539 - 1545.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Y. Li, J. P. Bernot, C. Illingworth, W. Lison, K. M. Bernot, W. B. Eggleston, K. J. Fogle, J. E. DiPaola, J. Kermicle, and M. Alleman
Gene Conversion Within Regulatory Sequences Generates Maize r Alleles With Altered Gene Expression
Genetics, December 1, 2001; 159(4): 1727 - 1740.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
P. Pichierri, A. Franchitto, R. Piergentili, C. Colussi, and F. Palitti
Hypersensitivity to camptothecin in MSH2 deficient cells is correlated with a role for MSH2 protein in recombinational repair
Carcinogenesis, November 1, 2001; 22(11): 1781 - 1787.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
H. M. Kearney, D. T. Kirkpatrick, J. L. Gerton, and T. D. Petes
Meiotic Recombination Involving Heterozygous Large Insertions in Saccharomyces cerevisiae: Formation and Repair of Large, Unpaired DNA Loops
Genetics, August 1, 2001; 158(4): 1457 - 1476.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. T. Tran, J. A. Simon, and R. M. Liskay
Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiae
PNAS, July 24, 2001; (2001) 161175998.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Elliott and M. Jasin
Repair of Double-Strand Breaks by Homologous Recombination in Mismatch Repair-Defective Mammalian Cells
Mol. Cell. Biol., April 15, 2001; 21(8): 2671 - 2682.
[Abstract] [Full Text]

Home page
 GeneticsHome page
J. A. Clikeman, S. L. Wheeler, and J. A. Nickoloff
Efficient Incorporation of Large (>2 kb) Heterologies Into Heteroduplex DNA: Pms1/Msh2-Dependent and -Independent Large Loop Mismatch Repair in Saccharomyces cerevisiae
Genetics, April 1, 2001; 157(4): 1481 - 1491.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
Q. Chen, A. Ijpma, and C. W. Greider
Two Survivor Pathways That Allow Growth in the Absence of Telomerase Are Generated by Distinct Telomere Recombination Events
Mol. Cell. Biol., March 1, 2001; 21(5): 1819 - 1827.
[Abstract] [Full Text]

Home page
 Genome Res.Home page
R. Friedman and A. L. Hughes
Gene Duplication and the Structure of Eukaryotic Genomes
Genome Res., March 1, 2001; 11(3): 373 - 381.
[Abstract] [Full Text]

Home page
 GeneticsHome page
C. H. Langley, B. P. Lazzaro, W. Phillips, E. Heikkinen, and J. M. Braverman
Linkage Disequilibria and the Site Frequency Spectra in the su(s) and su(wa) Regions of the Drosophila melanogaster X Chromosome
Genetics, December 1, 2000; 156(4): 1837 - 1852.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
E. Evans and E. Alani
Roles for Mismatch Repair Factors in Regulating Genetic Recombination
Mol. Cell. Biol., November 1, 2000; 20(21): 7839 - 7844.
[Full Text]

Home page
 Mol. Cell. Biol.Home page
P. T. Tran and R. M. Liskay
Functional Studies on the Candidate ATPase Domains of Saccharomyces cerevisiae MutLalpha
Mol. Cell. Biol., September 1, 2000; 20(17): 6390 - 6398.
[Abstract] [Full Text]

Home page
 GeneticsHome page
A. J. R. Bishop, E. J. Louis, and R. H. Borts
Minisatellite Variants Generated in Yeast Meiosis Involve DNA Removal During Gene Conversion
Genetics, September 1, 2000; 156(1): 7 - 20.
[Abstract] [Full Text]

Home page
 GeneticsHome page
A. Nicholson, M. Hendrix, S. Jinks-Robertson, and G. F. Crouse
Regulation of Mitotic Homeologous Recombination in Yeast: Functions of Mismatch Repair and Nucleotide Excision Repair Genes
Genetics, January 1, 2000; 154(1): 133 - 146.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
J. Wu, L. Gu, H. Wang, N. E. Geacintov, and G.-M. Li
Mismatch Repair Processing of Carcinogen-DNA Adducts Triggers Apoptosis
Mol. Cell. Biol., December 1, 1999; 19(12): 8292 - 8301.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. A. Nickoloff, D. B. Sweetser, J. A. Clikeman, G. J. Khalsa, and S. L. Wheeler
Multiple Heterologies Increase Mitotic Double-Strand Break-Induced Allelic Gene Conversion Tract Lengths in Yeast
Genetics, October 1, 1999; 153(2): 665 - 679.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Fabisiewicz and L. Worth Jr.
Escherichia coli MutS,L Modulate RuvAB-dependent Branch Migration between Diverged DNA
J. Biol. Chem., March 16, 2001; 276(12): 9413 - 9420.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. T. Tran, J. A. Simon, and R. M. Liskay
Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiae
PNAS, August 14, 2001; 98(17): 9760 - 9765.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the Genetics Society of America.