Suppressor Analysis of the Saccharomyces cerevisiae Gene REC104 Reveals a Genetic Interaction With REC102

Suppressor Analysis of the Saccharomyces cerevisiae Gene REC104 Reveals a Genetic Interaction With REC102

Laura Salem^a, Natalie Walter^b, and Robert Malone^b,a
^a Program in Genetics, University of Iowa, Iowa City, Iowa 52242
^b Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242

Corresponding author: Robert Malone, Department of Biological Sciences, 427 Biology Bldg., University of Iowa, Iowa City, IA 52242., robert-malone{at}uiowa.edu (E-mail)

Communicating editor: M. E. ZOLAN

REC104 is a gene required for the initiation of meiotic recombinationin Saccharomyces cerevisiae. To better understand the role ofREC104 in meiosis, we used an in vitro mutagenesis techniqueto create a set of temperature-conditional mutations in REC104and used one ts allele (rec104-8) in a screen for high-copysuppressors. An increased dosage of the early exchange geneREC102 was found to suppress the conditional recombinationalreduction in rec104-8 as well as in several other conditionalrec104 alleles. However, no suppression was observed for a nullallele of REC104, indicating that the suppression by REC102is not "bypass" suppression. Overexpression of the early meioticgenes REC114, RAD50, HOP1, and RED1 fails to suppress any ofthe rec104 conditional alleles, indicating that the suppressionmight be specific to REC102.

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