Genetic Analysis of hook, a Gene Required for Endocytic Trafficking in Drosophila

Corresponding author: Helmut Krämer, Center for Basic Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75235-9111., kramer{at}utsw.swmed.edu (E-mail)

Communicating editor: T. SCHÜPBACH

The Drosophila hook gene encodes a novel component of the endocytic compartment. Previously identified hook alleles, which still expressed truncated Hook proteins, affected the accumulation of internalized transmembrane ligands into multivesicular bodies (MVBs). To determine the hook null phenotype, we isolated nine new hook alleles on the basis of their characteristic hooked-bristle phenotype. At least one of these alleles, hk¹¹, is a complete loss-of-function allele. Flies carrying the hk¹¹ allele are viable and fertile but neither transmembrane ligands nor soluble ligands accumulate in MVBs. This effect on endocytosed ligands can be mimicked by the expression of Hook proteins truncated for the N- and C-terminal domains flanking the central coiled-coil region. The importance of all three domains for Hook function was confirmed by their conservation between two Drosophila and two human Hook proteins.

This article has been cited by other articles:

				A. Simonsen, R. C. Cumming, K. Lindmo, V. Galaviz, S. Cheng, T. E. Rusten, and K. D. Finley Genetic Modifiers of the Drosophila Blue Cheese Gene Link Defects in Lysosomal Transport With Decreased Life Span and Altered Ubiquitinated-Protein Profiles Genetics, June 1, 2007; 176(2): 1283 - 1297. [Abstract] [Full Text] [PDF]

				H. Sano, M. Ishino, H. Kramer, T. Shimizu, H. Mitsuzawa, C. Nishitani, and Y. Kuroki The Microtubule-binding Protein Hook3 Interacts with a Cytoplasmic Domain of Scavenger Receptor A J. Biol. Chem., March 16, 2007; 282(11): 7973 - 7981. [Abstract] [Full Text] [PDF]

				K. Wilhelmsen, M. Ketema, H. Truong, and A. Sonnenberg KASH-domain proteins in nuclear migration, anchorage and other processes J. Cell Sci., December 15, 2006; 119(24): 5021 - 5029. [Abstract] [Full Text] [PDF]

				A. L. Parks, J. R. Stout, S. B. Shepard, K. M. Klueg, A. A. Dos Santos, T. R. Parody, M. Vaskova, and M. A. T. Muskavitch Structure-Function Analysis of Delta Trafficking, Receptor Binding and Signaling in Drosophila Genetics, December 1, 2006; 174(4): 1947 - 1961. [Abstract] [Full Text] [PDF]

				A. A. Lanahan, T. W. Chittenden, E. Mulvihill, K. Smith, S. Schwartz, and M. Simons Synectin-dependent gene expression in endothelial cells Physiol Genomics, November 21, 2006; 27(3): 380 - 390. [Abstract] [Full Text] [PDF]

				C.-M. Lin, H.-J. Chen, C. L. Leung, D. A. D. Parry, and R. K. H. Liem Microtubule actin crosslinking factor 1b: a novel plakin that localizes to the Golgi complex J. Cell Sci., August 15, 2005; 118(16): 3727 - 3738. [Abstract] [Full Text] [PDF]

				E. A. Sevrioukov, N. Moghrabi, M. Kuhn, and H. Kramer A Mutation in dVps28 Reveals a Link between a Subunit of the Endosomal Sorting Complex Required for Transport-I Complex and the Actin Cytoskeleton in Drosophila Mol. Biol. Cell, May 1, 2005; 16(5): 2301 - 2312. [Abstract] [Full Text] [PDF]

				K. Luiro, K. Yliannala, L. Ahtiainen, H. Maunu, I. Jarvela, A. Kyttala, and A. Jalanko Interconnections of CLN3, Hook1 and Rab proteins link Batten disease to defects in the endocytic pathway Hum. Mol. Genet., December 1, 2004; 13(23): 3017 - 3027. [Abstract] [Full Text] [PDF]

				J. A. Fischer, S. Acosta, A. Kenny, C. Cater, C. Robinson, and J. Hook Drosophila Klarsicht Has Distinct Subcellular Localization Domains for Nuclear Envelope and Microtubule Localization in the Eye Genetics, November 1, 2004; 168(3): 1385 - 1393. [Abstract] [Full Text] [PDF]

				S. C. W. Richardson, S. C. Winistorfer, V. Poupon, J. P. Luzio, and R. C. Piper Mammalian Late Vacuole Protein Sorting Orthologues Participate in Early Endosomal Fusion and Interact with the Cytoskeleton Mol. Biol. Cell, March 1, 2004; 15(3): 1197 - 1210. [Abstract] [Full Text] [PDF]

				E. L. Rawlins, B. Lovegrove, and A. P. Jarman Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta Development, December 29, 2003; 130(26): 6475 - 6484. [Abstract] [Full Text] [PDF]

				A. S. Rawls and T. Wolff Strabismus requires Flamingo and Prickle function to regulate tissue polarity in the Drosophila eye Development, May 1, 2003; 130(9): 1877 - 1887. [Abstract] [Full Text] [PDF]

				I. Mendoza-Lujambio, P. Burfeind, C. Dixkens, A. Meinhardt, S. Hoyer-Fender, W. Engel, and J. Neesen The Hook1 gene is non-functional in the abnormal spermatozoon head shape (azh) mutant mouse Hum. Mol. Genet., July 1, 2002; 11(14): 1647 - 1658. [Abstract] [Full Text] [PDF]

				J. H. Walenta, A. J. Didier, X. Liu, and H. Kramer The Golgi-associated Hook3 Protein Is a Member of a Novel Family of Microtubule-binding Proteins J. Cell Biol., February 26, 2001; 152(5): 923 - 934. [Abstract] [Full Text] [PDF]

				R. Roepman, N. Bernoud-Hubac, D. E. Schick, A. Maugeri, W. Berger, H.-H. Ropers, F. P.M. Cremers, and P. A. Ferreira The retinitis pigmentosa GTPase regulator (RPGR) interacts with novel transport-like proteins in the outer segments of rod photoreceptors Hum. Mol. Genet., September 1, 2000; 9(14): 2095 - 2105. [Abstract] [Full Text] [PDF]

				C. P. Schonbaum, J. J. Perrino, and A. P. Mahowald Regulation of the Vitellogenin Receptor during Drosophila melanogaster Oogenesis Mol. Biol. Cell, February 1, 2000; 11(2): 511 - 521. [Abstract] [Full Text]

				J. Luzio, B. Rous, N. Bright, P. Pryor, B. Mullock, and R. Piper Lysosome-endosome fusion and lysosome biogenesis J. Cell Sci., January 5, 2000; 113(9): 1515 - 1524. [Abstract] [PDF]

				A. Parks, K. Klueg, J. Stout, and M. Muskavitch Ligand endocytosis drives receptor dissociation and activation in the Notch pathway Development, January 4, 2000; 127(7): 1373 - 1385. [Abstract] [PDF]

				M. Martin, S. J. Iyadurai, A. Gassman, J. G. Gindhart Jr., T. S. Hays, and W. M. Saxton Cytoplasmic Dynein, the Dynactin Complex, and Kinesin Are Interdependent and Essential for Fast Axonal Transport Mol. Biol. Cell, November 1, 1999; 10(11): 3717 - 3728. [Abstract] [Full Text]

				A. Sunio, A. B. Metcalf, and H. Krämer Genetic Dissection of Endocytic Trafficking in Drosophila Using a Horseradish Peroxidase-Bride of Sevenless Chimera: hook Is Required for Normal Maturation of Multivesicular Endosomes Mol. Biol. Cell, April 1, 1999; 10(4): 847 - 859. [Abstract] [Full Text]