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Genetics, Vol. 151, 439-446, February 1999, Copyright © 1999

Escherichia coli MutM Suppresses Illegitimate Recombination Induced by Oxidative Stress

Masaaki Ondaa, Katsuhiro Hanadaa, Hirokazu Kawachia, and Hideo Ikedaa
a Department of Molecular Biology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan

Corresponding author: Hideo Ikeda, Department of Molecular Biology, Institute of Medical Science, University of Tokyo, P.O. Takanawa, Tokyo 108-8639, Japan., ike{at}ims.u-tokyo.ac.jp (E-mail)

Communicating editor: R. MAURER

DNA damage by oxidative stress is one of the causes of mutagenesis. However, whether or not DNA damage induces illegitimate recombination has not been determined. To study the effect of oxidative stress on illegitimate recombination, we examined the frequency of {lambda}bio transducing phage in the presence of hydrogen peroxide and found that this reagent enhances illegitimate recombination. To clarify the types of illegitimate recombination, we examined the effect of mutations in mutM and related genes on the process. The frequency of {lambda}bio transducing phage was 5- to 12-fold higher in the mutM mutant than in the wild type, while the frequency in the mutY and mutT mutants was comparable to that of the wild type. Because 7,8-dihydro-8-oxoguanine (8-oxoG) and formamido pyrimidine (Fapy) lesions can be removed from DNA by MutM protein, these lesions are thought to induce illegitimate recombination. Analysis of recombination junctions showed that the recombination at Hotspot I accounts for 22 or 4% of total {lambda}bio transducing phages in the wild type or in the mutM mutant, respectively. The preferential increase of recombination at nonhotspot sites with hydrogen peroxide in the mutM mutant was discussed on the basis of a new model, in which 8-oxoG and/or Fapy residues may introduce double-strand breaks into DNA.




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