Genetics, Vol. 150, 1419-1428, December 1998, Copyright © 1998

The SFP1 Gene Product of Saccharomyces cerevisiae Regulates G2/M Transitions During the Mitotic Cell Cycle and DNA-Damage Response

Zhiheng Xua and David Norrisa,b
a Waksman Institute of Microbiology, Piscataway, New Jersey 08854-8020
b Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854-8020

Corresponding author: David Norris, Waksman Institute of Microbiology, 190 Frelinghuysen Rd., Piscataway, NJ 08854-8020., norris{at}mbcl.rutgers.edu (E-mail).

Communicating editor: F. WINSTON

In eukaryotic cells, checkpoint pathways arrest cell-cycle progression if a particular event has failed to complete appropriately or if an important intracellular structure is defective or damaged. Saccharomyces cerevisiae strains that lack the SFP1 gene fail to arrest at the G2 DNA-damage checkpoint in response to genomic injury, but maintain their ability to arrest at the replication and spindle-assembly checkpoints. sfp1{Delta} mutants are characterized by a premature entrance into mitosis during a normal (undamaged) cell cycle, while strains that overexpress Sfp1p exhibit delays in G2. Sfp1p therefore acts as a repressor of the G2/M transition, both in the normal cell cycle and in the G2 checkpoint pathway. Sfp1 is a nuclear protein with two Cys2His2 zinc-finger domains commonly found in transcription factors. We propose that Sfp1p regulates the expression of gene products involved in the G2/M transition during the mitotic cell cycle and the DNA-damage response. In support of this model, overexpression of Sfp1p induces the expression of the PDS1 gene, which is known to encode a protein that regulates the G2 checkpoint.




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